Effect of mitochondrial dysfunction on the apoptosis of retinal ganglion cells in glaucoma
10.13389/j.cnki.rao.2024.0115
- VernacularTitle:线粒体功能障碍在青光眼视网膜神经节细胞凋亡中的作用
- Author:
Xi ZHANG
1
;
Chunqiong DONG
;
Yongjin ZHANG
;
Liyang WANG
;
Yun ZHANG
;
Fei YUAN
;
Jinhui DAI
Author Information
1. 201104 上海市,上海市老年医学中心眼科
- Keywords:
mitochondrial function;
apoptosis;
mitochondrial membrane potential;
reactive oxygen species;
intraoc-ular pressure
- From:
Recent Advances in Ophthalmology
2024;44(8):602-606
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mitochondrial dysfunction in glaucoma-related optic nerve injury and its effect on the apoptosis of retinal ganglion cells(RGCs).Methods The RGC-5 cells were incubated under elevated pressure(70 mmHg,1 kPa=7.5 mmHg)on the basis of standard atmospheric pressure(100 kPa)for 12 h,24 h,and 48 h,respective-ly.The RGC-5 cells cultured under the standard atmospheric pressure were selected as the blank group.The apoptosis of RGC-5 cells was detected through Annexin V/PI staining.The mitochondrial membrane potential(MMP)and reactive oxy-gen species(ROS)level were measured using the JC-1 staining and DCFH-DA fluorescent probe.The expression levels of B cell lymphoma-2(BCL-2)protein,BCL-2-associated X(BAX)protein,cleaved Caspase-3,and cytochrome C(CytC)pro-tein were detected using Western blot.Results Compared with the blank group,the MMP in the 12 h,24 h and 48 h groups significantly decreased(all P＜0.05).Compared with the blank group,the cell apoptosis and ROS level in the 24 h and 48 h groups significantly increased(all P＜0.05),while there was no statistically significant difference in the cell apop-tosis and ROS level between the 12 h group and the blank group(both P＞0.05).Compared with the blank group,the rela-tive expression levels of cleaved Caspase-3 and BAX protein in the 24 h and 48 h groups significantly increased(all P＜0.05),while the relative expression levels of cleaved Caspase-3 and BAX protein in the 12 h group showed no significant difference(both P＞0.05).Compared with the blank group,the relative expression level of BCL-2 protein in the 12 h,24 h and 48 h groups decreased,and the differences were statistically significant(all P＜0.05).Compared with the blank group,the relative expression level of Cyt C protein in the 48 h group significantly increased(P＜0.05),while the relative expression level of Cyt C protein in the 12 h and 24 h groups showed no significant difference(both P＞0.05).Conclu-sion After culture at 70 mmHg pressure,RGC-5 cells show increased apoptosis and mitochondrial dysfunction.The mito-chondrial damage at the early stage of stress injury may be closely related to exogenous apoptosis pathways.
