Effects of monocular deprivation during critical period of visual development on astrocytes in different regions of the mouse brain
10.13389/j.cnki.rao.2024.0099
- VernacularTitle:视觉发育关键期单眼剥夺对小鼠大脑不同区域星形胶质细胞的影响
- Author:
Yifan SUN
1
,
2
;
Xiao WANG
;
Shiqiao YANG
;
Kailei WANG
;
Xuechun WANG
;
Yamin CHEN
;
Xiaoran ZHANG
;
Panpan LÜ
;
Rui HAO
Author Information
1. 300020 天津市,天津医科大学眼科临床学院
2. 300020 天津市,天津市眼科医院
- Keywords:
monocular deprivation;
visual cortex;
superior colliculus;
hippocampus;
astrocyte
- From:
Recent Advances in Ophthalmology
2024;44(7):517-521
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of monocular deprivation(MD)on the expression of astrocytes in the superior colliculus,hippocampus,and visual cortex in mice during the critical period of visual development.Methods Eighteen C57BL/6J mice were randomly divided into the normal control group(CON group)and the MD group,with 9 mice in each group.Mice were bred under the 12 h/12 h dark/light conditions.Mice in the CON group received no treat-ment,while mice in the MD group underwent MD of the right eye on postnatal day 27,and the tissue was removed after 7 days.The mRNA and protein expression levels of glial fibrillary acidic protein(GFAP)in the superior colliculus,hippo-campus and visual cortex of mice in the two groups were detected using the real-time reverse transcription quantitative pol-ymerase chain reaction(RT-qPCR)and Western blot,respectively.The number of astrocytes labeled by GFAP and central nervous system specific protein β(S100β)in the superior colliculus,hippocampus and visual cortex of mice in the two groups was detected using the immunofluorescence staining.Results RT-qPCR and Western blot results showed that compared with the CON group,the mRNA and protein expression levels of GFAP in the superior colliculus,hippocampus(CA1,CA3 and dentate gyrus)and visual cortex of mice in the MD group decreased,and the differences were statistically significant(all P＜0.05).The immunofluorescence staining results showed that compared with the CON group,the number of GFAP and S1OOβ co-labeled astrocytes in the superior colliculus,hippocampus(CA1,CA3 and dentate gyrus)and visual cortex of mice in the MD group decreased,and the differences were statistically significant(all P＜0.05).Conclusion MD of mice during the critical period of visual development can result in a decrease in the number of astrocytes in the supe-rior colliculus,hippocampus and visual cortex.
