Correlation of Toll-like receptor and nuclear factor-KB levels in monocytes with type 2 diabetic autonomic neuropathy
10.7683/xxyxyxb.2024.08.013
- VernacularTitle:单核细胞Toll样受体和核因子-κB水平与2型糖尿病患者自主神经病变的相关性
- Author:
Jing GUO
1
;
Weisheng SHI
Author Information
1. 濮阳市人民医院内分泌科,河南 濮阳 457000
- Keywords:
type 2 diabetes mellitus;
autonomic neuropathy;
monocyte;
Toll-like receptor;
nuclear factor-KB
- From:
Journal of Xinxiang Medical College
2024;41(8):772-775,782
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the correlation between Toll-like receptor(TLR)and nuclear factor-KB(NF-κB)levels in monocytes and type 2 diabetic autonomic neuropathy(T2DAN).Methods A total of 112 patients with type 2 diabetes mellitus(T2DM)admitted to the Puyang People's Hospital from April 2021 to December 2022 were selected as the observation group,and 50 healthy volunteers who underwent physical examination in the hospital during the same period were selected as the control group.Reverse transcription polymerase chain reaction(RT-PCR)was used to detect plasma TLR2,TLR4 and NF-κB levels in monocytes of patients in the two groups.According to the presence or absence of autonomic neuropathy,patients in the observation group were divided into the autonomic neuropathy group(n=46)and the non-autonomic neuropa-thy group(n=66),and clinical data of patients in these two groups were compared.Multivariate logistic regression analysis was used on indicators with statistically significant differences to determine the risk factors for T2DAN,and Spearman correlation analysis was used to evaluate the correlation between TLR2,TLR4 and NF-κB levels and T2DAN.Results The TLR2,TLR4 and NF-κB levels in monocytes of patients in the observation group were higher than those in the control group(P＜0.05).Univariate analysis showed that there were significant differences in age,monocyte/high-density lipoprotein ratio(MHR),and levels of cystatin C,TLR2,TLR4 and NF-κB between the autonomic neuropathy group and the non-autonomic neuropathy group(P＜0.05).Multivariate logistic regression analysis showed that MHR,TLR2,TLR4 and NF-κB levels were risk factors for autonomic neuropathy in T2DM patients(P＜0.05).Spearman correlation analysis showed that TLR2,TLR4 and NF-κB levels were positively correlated with T2 DAN(r=0.825,0.822,0.819;P＜0.05).Conclusion The expressions of TLR2,TLR4 and NF-κB are up-regulated in T2DM patients,and the expression levels of TLR2,TLR4 and NF-κB are correlated with autonomic neuropathy in T2DM patients.
