Clinical sesearch study on improving the positive rate of peritoneal dialysis-related peritonitis
10.3760/cma.j.issn.1674-2907.2019.33.006
- VernacularTitle:提高腹膜透析相关性腹膜炎培养阳性率方法探究
- Author:
Jun YANG
1
;
Lijuan ZHAO
;
Guoshuang XU
;
Rong LI
;
Fanfan DONG
Author Information
1. 空军军医大学西京医院肾脏内科
- Keywords:
Dialysis;
Peritoneal dialysis;
Peritonitis;
Culture method
- From:
Chinese Journal of Modern Nursing
2019;25(33):4287-4290
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore methods of culturing peritonealdialysate pathogen in patients with peritoneal dialysisrelated peritonitis, providing reliable basis for more effective clinical treatment. Methods 235 patients with peritoneal dialysis related peritonitis were selected from the Department of Nephrology of Xijing Hospital Affiliated to the Air Force Medical University from January 2015 to December 2018, and they were randomly divided into four groups. Group A (traditional group,n=58): 10 ml peritoneal dialysate were directly extracted and then injected into blood bottle culture. Group B (suspension group,n=58):10 ml extracted peritoneal dialysate were placed for 1 hour then injected into blood bottle culture. Group C (centrifugation group,n=59): 50 ml extracted peritoneal dialysate were centrifuged for 15 min, and whose supernatant sediment was suspended by 5 ml sodium chloride solution of 9% and then injected into the blood culture bottle. Group D (suspension+ centrifugation group,n=60): After the dialysate were given to patients and placed for 1 hour, 50 ml peritoneal dialysate was extracted and centrifuged for 15 min, and whose supernatant sediment was suspended by 5 ml sodium chloride solution of 9% and then injected into the blood culture bottle. The positive culture rates were observed and compared in these four groups. Results There was a statistically significance in the differences of the positive culture rates of the 4 groups, which were 55.2 %, 70.7 %, 76.3 %, and 86.7 %, respectively(χ2=15.233,P＜ 0.01). Further pairwise comparison indicated that the positive culture rates of centrifuge culture group and suspension + centrifuge culture group was significantly higher than that of traditional culture group(χ2=5.787, 14.262;P＜ 0.01). Conclusions The positive culture rate of peritoneal dialysate pathogen would be increased through the culturing method of suspension + centrifugation, providing basis for the subsequent treatment and estimation of peritonitis.
