Homing and characteristic analysis of macrophage in immune-mediated aplastic anemia model mice
10.3760/cma.j.cn121090-20230927-00142
- VernacularTitle:免疫介导的再生障碍性贫血模型小鼠巨噬细胞归巢及特征分析
- Author:
Wei SUN
1
;
Zenghua LIN
;
Han WANG
;
Hui JIA
;
Laigen TONG
;
Zhipeng ZHANG
;
Wen LI
;
Chengcheng ZHOU
;
Hong LIU
Author Information
1. 南通大学医学院，南通 226001
- From:
Chinese Journal of Hematology
2024;45(6):594-598
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the dynamic homing process and characteristics of macrophages in different organs of immune-mediated aplastic anemia (AA) model mice. Macrophages in donor lymph nodes were sorted by magnetic beads and labeled with PKH67. After modeling according to the preparation method of the AA model, peripheral blood rountine analysis, bone marrow biopsy and HE staining results were analyzed to verify the modeling effect. On days 4, 8, and 12 of modeling, the bone marrow, spleen, and lymph node mononuclear cells were collected, and dynamic changes of PKH67-labeled macrophages in donor mice were analyzed by flow cytometry. In this study, dynamic changes in PKH67-labeled macrophages in the pathogenesis of AA model mice were explored. Macrophages in donor mice homed to the lymph nodes, expanding and differentiating in the lymph nodes, and finally transported to the bone marrow and spleen. Through proteomics mass spectrometry analysis, the related immune inflammatory response pathway of macrophages involved in the activation of the AA bone marrow microenvironment was preliminarily revealed, which provides a basis for the pathological macrophages involved in the pathogenesis of AA model mice.
