Clinical study of the cytokine panel in the diagnosis of ocular chronic graft-versus-host disease
10.3760/cma.j.cn121090-20231031-00242
- VernacularTitle:细胞因子组合辅助诊断眼部慢性移植物抗宿主病的临床研究
- Author:
Xianjing CHENG
1
;
Rui JI
;
Ruihao HUAN
;
Shiqin HUANG
;
Wei FAN
;
Yuancheng ZHAO
;
Rongdi YUAN
;
Xiaoqi WANG
;
Xi ZHANG
Author Information
1. 陆军军医大学第二附属医院血液病医学中心，全军临床重点专科，创伤与化学中毒国家重点实验室，重庆市医学重点学科，血液病与微环境重庆市重点实验室，重庆 400037
- Keywords:
Allogeneic hematopoietic stem cell transplantation;
Ocular cGVHD;
Tear fluid;
Cytokine;
Biomarker
- From:
Chinese Journal of Hematology
2024;45(3):242-248
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the association between cytokines and ocular chronic graft-versus-host disease (cGVHD) and identify specific biomarkers for ocular cGVHD to enhance clinical diagnosis, treatment, and evaluation.Methods:A mouse model of cGVHD was established to explore the correlation between cGVHD and serum cytokines. Based on the findings from the animal experiments and literature review, a panel of 16 cytokine combinations was identified. Enzyme-linked immunosorbent assay (ELISA) was used to compare the cytokine concentrations in the serum and tear samples from patients who underwent allogeneic hematopoietic stem cell transplantation from June 2017 to March 2022 at the Medical Center of Hematology, Xinqiao Hospital, Army Medical University.Results:① Compared with the control group, mice with cGVHD exhibited elevated serum IL-1β, IL-6, IL-8, IL-17, IFN-γ, CX3CL1, CXCL11, CXCL13, CCL11, and CCL19 concentrations (all P<0.05). ② Analysis of the cytokine profiles of the serum and tear samples revealed that compared with patients without ocular cGVHD, those with ocular cGVHD exhibited increased serum IL-8 [ P=0.032, area under the curve (AUC) =0.678]; decreased serum IL-10 ( P=0.030, AUC=0.701) ; elevated IL-8, IFN-γ, CXCL9, and CCL17 in tear samples; and lower IL-10 and CCL19 in tear samples (all P<0.05, all AUC>0.7). Moreover, cytokines in tear samples showed correlations with ocular surface parameters related to ocular cGVHD. Conclusions:Tear fluid demonstrates greater specificity and sensitivity as a biomarker for diagnosing ocular cGVHD than serum biomarkers. Among the identified cytokines in tear samples, IL-8, IL-10, IFN-γ, CXCL9, CCL17, and CCL19 serve as diagnostic biomarkers for ocular cGVHD post-transplantation, offering practical reference value for diagnosis.
