Differential analysis of the structure and distribution of gut microbiota between children with cow's milk protein allergy and healthy children in Xiamen,Fujian,China
10.3760/cma.j.issn.1673-4912.2024.07.007
- VernacularTitle:福建厦门地区牛奶蛋白过敏患儿与健康儿童间肠道菌群结构和分布的差异分析
- Author:
Ying SU
1
;
Guanhong WU
;
Mingzhen LI
;
Xiaomei HUANG
;
Jingfang CHEN
Author Information
1. 复旦大学附属儿科医院厦门医院（厦门市儿童医院）消化内科 361006
- Keywords:
Cow's milk protein allergy;
Children;
Gut microbiota;
Microbial community structure;
Metabolic pathways
- From:
Chinese Pediatric Emergency Medicine
2024;31(7):512-517
- CountryChina
- Language:Chinese
-
Abstract:
Objective:There are significant differences in the distribution of gut microbiota among different populations in different regions.This study mainly explores the structure and distribution of gut microbiota between cow's milk protein allergy（CMPA）children and healthy children in Xiamen,Fujian,and further explores the correlation between gut microbiota and the occurrence and development of CMPA.Methods:Fecal samples have been collected from 30 children with CMPA diagnosed at Xiamen Children’s Hospital from June 2022 to March 2023,and fecal samples from 30 healthy children of the same age have also been collected.The structure and distribution of gut microbiota between the two groups were analyzed using 16S rDNA gene sequencing and bioinformatics analysis.Results:The structure and distribution of gut microbiota between CMPA children and healthy children were quite different.There were no significant differences in α diversity index between groups（ P>0.05）.The analysis of similarities test results showed that there was a significant difference in the distribution of gut microbiota between two groups（ R=0.037, P<0.05）.The β diversity index from PCA showed that there was a significant difference in bacterial community structure between two groups（ P<0.05）.Compared with healthy children,the abundance of Akkermansia in CMPA children was significantly decreased and the abundance of Corynebacterium was significantly increased in CMPA children.LEfSe showed that Proteobacteria were mainly enriched in CMPA children,while Lachnospiraceae were mainly enriched in healthy children.In addition,KEGG analysis showed that the top enrichment of 20 pathways,such as Fructose and mannose metabolism,was significantly decreased in CMPA children（ P<0.05）.COG analysis showed that the enrichment of pathways such as Galactose mutarotase and related enzymes was significantly decreased in CMPA children（ P<0.05）,while the enrichment of pathways including Predicted metal-sulfur cluster biosynthetic enzyme and other two pathways was significantly increased（ P<0.05）. Conclusion:There are significant differences in the structure and distribution of gut microbiota between CMPA children and healthy children,suggesting that a strong correlation between the occurrence and development of CMPA and the gut microbiota.
