Abnormal expression of WWP1 in chronic lymphocytic leukemia and its clinical significance
10.3760/cma.j.issn.0253-2727.2020.09.006
- VernacularTitle:WWP1在慢性淋巴细胞白血病中的异常表达及临床意义
- Author:
Changqing YANG
1
;
Lei CAO
;
Rong HUANG
;
Hailing LIU
;
Yujie WU
;
Chun QIAO
;
Wei XU
;
Jianyong LI
;
Lei FAN
Author Information
1. 南京医科大学第一附属医院，江苏省人民医院血液科，南京 210029
- Keywords:
Chronic lymphocytic leukemia;
Reverse transcription polymerase chain reaction;
E3 ubiquitin ligase;
Prognosis
- From:
Chinese Journal of Hematology
2020;41(9):737-742
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aims to investigate the expression of E3 ubiquitin-ligase (WWP1) in chronic lymphocytic leukemia (CLL) patients and analyze its correlation with clinical prognostic indicators (TP53, CD38, IGHV mutation) and its prognostic value.Methods:A total of 48 CLL patients and 9 age-matched normal subjects were enrolled in the study. The WWP1 expression was detected by SYBR Green-based real-time PCR, and the clinical relationship was analyzed by GraphPad Prism software.Results:The WWP1 median expression was 0.007 (95% CI 0.005-0.010) in the normal control group and 0.031 (95% CI 0.019-0.044) in the CLL group ( P<0.001) . A sub-groups analysis implicated a statistically significant result ( P=0.022) , showing that the median time from a relatively high and low transcription level of WWP1 to the first treatment was 24 months and 35 months, respectively. Positive CD38 and ZAP-70 expressions were associated with a higher WWP1 expression ( P=0.012 and 0.029, respectively) . Conclusion:An abnormal WWP1 mRNA expression was found in CLL patients with significant correlation with ZAP-70 and CD38 expressions, and WWP1 may become a new supplement of CLL prognostic markers.
