Comparison of immune reconstitution at early stage after unmanipulated haploidentical stem cell transplantation between high-and standard-risk Philadelphia chromosome-negative acute lymphoblastic leukemia patients in CR1
10.3760/cma.j.issn.0253-2727.2016.08.004
- VernacularTitle:高危和标危Ph阴性急性淋巴细胞白血病非体外去T细胞单倍型造血干细胞移植后早期免疫重建的比较研究
- Author:
Zhilei BIAN
1
;
Yingjun CHANG
;
Lanping XU
;
Yu WANG
;
Xiaohui ZHANG
;
Kaiyan LIU
;
Xiaojun HUANG
Author Information
1. 北京市造血干细胞移植重点实验室
- Keywords:
Leukemia,lymphoid;
Philadelphia chromosome;
Hematopoietic stem cell transplantation;
Immune reconstitution;
Lymphocyte subsets
- From:
Chinese Journal of Hematology
2016;37(8):650-655
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the early stage immune reconstitution of high-and standardrisk Philadelphia chromosome-negative acute lymphoblastic leukemia (ALL) CR1 patients who had haploidentical blood and marrow stem cell transplantation (HBMT).Methods A total of 49 Ph-negative ALL CR1 patients who received HBMT and had complete early stage immune reconstitution data(+30,+60 and +90 d post transplantation) from Jan.2010 to Dec.2012 were enrolled.Immunophenotyping for B and T lymphocytes was performed post HBMT via flow cytometry.Fresh peripheral blood cells were stained with fluorochrome-labeled monoclonal antibodies against cluster of differentiation CD19,CD3,CD4,CD8,CD45RO,CD45RA and CD28.The early reconstitution of lymphocyte subsets,survival and prognosis between standard-risk group,high-risk adult group and high-risk children group were compared.Results There were no significant differences in all these T lymphocyte subsets among three groups at the three check points (P＞0.05).Moreover,at the same time,comparable outcome had been achieved between standard-risk group (n=18),high-risk adult group (n=16) and high-risk children group (n=15).There were no differences in 2-y relapse incidence (27.8％ vs 31.3％ vs 26.7％,P=0.957),2-y non-relapse mortality (11.1％ vs 0 vs 13.3％,P=0.185),2-y leukemia free survival (61.1％ vs 68.8％ vs 60.0％,P=0.834) and overall survival (77.8％ vs 68.8％ vs 60.0％,P=0.529) among the three groups.Incidence of grade Ⅱ-Ⅳ aGVHD was 44.4％ vs 12.5％ vs 46.7％(P=0.075) and incidence of cGVHD was 61.1％ vs 50.0％ vs 40.0％ (P=0.249).Conclusion Comparison of immune reconstitution at early stage may be a reasonable cause to explain that equivalent outcomes were observed among high-and standard-risk Ph-negative ALL CR1 patients after HBMT.
