Study on the relationship between the expression levels of serum LncRNA MALAT1 and miR-146a and the balance of Th1/Th2 and Th17/Treg immune cells in patients with inflammatory bowel disease
10.3969/j.issn.1672-2159.2024.08.009
- VernacularTitle:血清LncRNA MALAT1、miR-146a表达水平与炎症性肠病患者Th1/Th2、Th17/Treg免疫细胞平衡的关系研究
- Author:
Yue SHEN
1
;
Jiaxin WANG
;
Li LI
Author Information
1. 721000 宝鸡高新医院消化科
- Keywords:
Inflammatory bowel disease;
LncRNA MALAT1;
miR-146a;
Th1/Th2;
Th17/Treg
- From:
Modern Interventional Diagnosis and Treatment in Gastroenterology
2024;29(8):932-936
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between the expression levels of serum long-chain non-coding ribonucleic acid lung cancer metastasis-associated transcript 1(LncRNA MALAT1)and microRNA-146a(miR-146a)and the balance of helper T cell(Th)1/Th2 and helper T cell 17(Th17)/regulatory T cell(Treg)immune cells in patients with inflammatory bowel disease(IBD).Methods IBD patients diagnosed and treated in our hospital from June 2021 to June 2023 were selected as observation group(n=151),patients were divided into active group(n=78)and the remission group(n=73)according to the disease activity,healthy subjects in our hospital during the same period were selected as control group(n=102).The expression levels of serum LncRNA MALAT1 and miR-146a were detected by real-time fluorescence quantitative polymerase chain reaction(QRT-PCR).The proportions of Th1,Th2,Th17 and Treg cells in peripheral blood were detected by flow cytometry,and the ratios of Th1/Th2 and Th17/Treg were calculated,Th1/Th2 and Th17/Treg related cytokines[interleukin(IL)-6,tumor necrosis factor-β(TNF-[3),interferon-γ(IFN-γ),interleukin(1L)-10,interleukin(IL)-17,interleukin(IL)-22]were detected by enzyme-linked immunosorbent assay(ELISA).The relationship between the expression levels of LncRNA MALAT1 and miR-146a and the balance of Th1/Th2 and Th17/Treg immune cells in IBD patients was analyzed by Pearson correlation analysis.Results The expression levels of serum LncRNA MALAT1,miR-146a,Th1,Th1/Th2,IFN-γ,IL-6,Th17,Th17/Treg,IL-17 and IL-22 in three groups were compared,active group＞remission group＞control group(P＜0.05).Th2,TNF-β,Treg and IL-10 in three groups were compared,active group＜remission group＜control group(P＜0.05).Pearson correlation analysis showed that,the expression levels of serum LncRNA MALAT1 and miR-146a were positively correlated with Th1,Th1/Th2,IFN-γ,IL-6,Th17,Th17/Treg,IL-17 and IL-22(P＜0.05),and negatively correlated with Th2,TNF-β,Treg and IL-10(P＜0.05).Conclusion Serum LncRNA MALAT1 and miR-146a are significantly up-regulate in patients with active IBD,and the expression levels are closely relate to the balance of Th1/Th2 and Th17/Treg immune cells,suggesting that they may affect the occurrence and development of IBD by regulating immune balance.
