Effect of Acacetin on Inhibition of Apoptosis in Helicobacter pylori-Infected Gastric Epithelial Cell Line GES-1
10.3969/j.issn.1672-2159.2024.03.011
- VernacularTitle:金合欢素对幽门螺杆菌感染的胃上皮GES-1细胞凋亡的抑制作用
- Author:
Qi-Xi YAO
1
;
Zi-Yu LI
;
Hou-Le KANG
;
Xin HE
;
Min KANG
Author Information
1. 646000 西南医科大学附属医院消化内科
- Keywords:
Gastric epithelial GES-1 cells;
Helicobacter pylori;
infection;
Acacetin;
Apoptosis
- From:
Modern Interventional Diagnosis and Treatment in Gastroenterology
2024;29(3):307-311
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aims to elucidate the protective role of Acacetin against apoptosis in HP-infected GES-1 cells and to delineate its potential underlying mechanisms.Materials and Methods GES-1 cells were subjected to in vitro treatment with HP and Acacetin.Cell viability was assessed utilizing the CCK-8 assay,alterations in cell migration and healing capacities through the wound healing assay,rates of apoptosis via flow cytometry,and expression levels of apoptosis-associated proteins through western blot analysis.Results HP infection led to a diminution in GES-1 cell viability,a suppression of cell migration,an augmentation in the rate of apoptosis,and an increase in the expression levels of Bax and cle-caspase3.Conversely,treatment with Acacetin was found to enhance cell viability,mitigate apoptosis induced by HP infection,and modulate the expression of apoptosis proteins by downregulating Bax and cle-caspase3.Discussion and Conclusion Acacetin significantly improves GES-1 cell vitality and inhibits apoptosis in HP-infected GES-1 cells,thereby offering a protective effect on gastric mucosal epithelial cells.
