β-elemene enhances aclarubicin-induced apoptotic effect in HL-60 cells and its mechanism
10.3760/cma.j.issn.0253-2727.2009.12.007
- VernacularTitle:β-榄香烯增强阿克拉霉素对HL-60细胞诱导凋亡作用及其机制研究
- Author:
Cui-Ping ZHENG
1
;
Xiang-Min TONG
;
Hang-Ping YAO
;
Jun YANG
;
Jie XU
;
Xiao-Ping CAI
;
Zheng LIU
Author Information
1. 温州市第二人民医院
- Keywords:
Elemene;
Aclarubicin;
HL-60 cells;
Apoptosis
- From:
Chinese Journal of Hematology
2009;30(12):821-824
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of β-elemene combined with aclarubicin on the induction of HL-60 cell apoptosis and its mechanisms in antileukemia therapy.Methods HL-60 cells were treated for 20 hours with different dose of aclarubicin(0.05,0.10,0.25 μg/ml) or with different concentrations of β-elemene (10,20,40 μg/ml) in the presence or absence of aclarubicin (0.10 μg/m).The apoptotic rate was analyzed by flow cytometry(FCM),the productions of PGE2 in culture supernatants was detected by competitive ELISA and the expressions of COX-2 and NF-kappaB activitv in HL-60 cells by Western blot.Results Lower concentration of aclarubicin (0.05,0.10 μg/ml) didn't affect apoptotic rate,and COX-2,NF-kappa B and PGE2 expression on HL-60 cells.Combined treatment of β-elemene and aclarubicin (0.10 μg/ml) enhanced the apoptotic effect and down-regulated COX-2,NF-kappaB and PGE2 expressions.There was a positive correlation between the effects and β-elemene concentrations.Conclusion β-elemene enhances aclarubicin-mediated apoptotic effect,down-regulation of COX-2 and their inducing products PGE2 in HL-60 cells by suppressing activitation of NF-kappaB.
