Study on induction of leukemic cell apoptosis by antisense oligodeoxynucleotide of human telomerase RNA

VernacularTitle:端粒酶RNA的反义寡核苷酸诱导白血病细胞凋亡的研究
Author: Wanzhuo XIE ¹ ; Maofang LIN
Author Information

1. 浙江大学附属第一医院
From: Chinese Journal of Hematology 2001;22(5):245-248
CountryChina
Language:Chinese
Abstract: Objective　To explore the antitumor activity of human telomerase RNA antisense oligodeoxynucleotide (As-ODN) to leukemic cells and its mechanisms. Methods　As-ODN was transfected into HL-60 cells by liposomal transfection. Telomerase activity of HL-60 cells was examined by telomeric repeat amplification protocol (TRAP)-enzyme-linked immunosorbent assay(ELISA). Apoptosis was analyzed by morphology, DNA agarose gel electrophoresis and flow cytometry. Results　The telomerase activity in HL-60 cells, being transfected with 0.1～2.0 μmol/L of As-ODN for 1～6 days, varied from 1.043±0.045 to 0.063±0.011 in a dose- and time-dependent manner. The proliferation of As-ODN-transfected HL-60 cells was lower than that of control, and the cells underwent apoptosis. The Ms-ODN(missense ODN)-transfected HL-60 cells didn′t show these changes. Conclusion　As-ODN can inhibit the telomerase activity of HL-60 cells specifically and induce apoptosis.