Involvement of mitochondrial membrane potential in the homoharringtonine induced apoptosis of leukemic T-cells
10.3760/j:issn:0253-2727.2001.05.004
- VernacularTitle:线粒体膜电位在高三尖杉酯碱诱导T-淋巴细胞白血病细胞Molt-3凋亡中的作用
- Author:
Zhen CAI
1
;
Maofang LIN
;
Ludwig WOLFDIETER
;
Karawajew LEONID
Author Information
1. 浙江大学附属第一医院
- From:
Chinese Journal of Hematology
2001;22(5):238-240
- CountryChina
- Language:Chinese
-
Abstract:
Objective Investigation of the role of mitochondrial membrane potential (MMP) in the homoharringtonine (HHT)-induced apoptosis. Methods Annexin V staining, flow cytometry and confocal laser scan microscopy were used to observe the relationship between Bax, cytochrome C and MMP in the HHT-induced apoptosis of leukemic T lymphocytic line Molt-3. Results The induction of apoptosis by HHT resulted in the translocation of Bax from cytosol to mitochondrial membrane and the decrease of cellular MMP, followed by the release of cytochrome C from mitochondria to cytosol. Conclusions Changes of mitochondrial membrane potential might play a critical role in the HHT-induced apoptosis of leukemic T-cells.
