Comparison of biological characteristics of adipose-derived stem cells in young and old mice
- VernacularTitle:年轻和老年小鼠脂肪来源干细胞生物学特性对比
- Author:
Meiyu LIN
1
,
2
,
3
;
Xiang YAO
;
Jing GAO
;
Xilong ZHAO
;
Xinghua PAN
;
Guangping RUAN
Author Information
- Keywords: adipose-derived stem cell; adipose tissue; young; old age; mouse; anti-aging
- From: Chinese Journal of Tissue Engineering Research 2025;29(19):4063-4068
- CountryChina
- Language:Chinese
- Abstract: BACKGROUND:Adipose-derived stem cells have anti-aging effects,but whether adipose-derived stem cells from donors of different ages are different needs further study. OBJECTIVE:To compare the biological properties of adipose-derived stem cells in old and young mice. METHODS:Adipose-derived stem cells were extracted from adipose tissue of C57BL mice aged 8 and 14 weeks,respectively.The differences of cell cycle,apoptosis,and proliferation of adipose-derived stem cells in old and young mice were compared.The expression levels of aging-related P21 and P27 genes and proteins of adipose-derived stem cells in old and young mice were detected by quantitative PCR and western blot assay. RESULTS AND CONCLUSION:Compared with old mouse adipose-derived stem cells,young mouse adipose-derived stem cells were more active,more regular in morphology,less apoptosis,faster proliferation,and lower in expression of age-related P21 and P27 genes and proteins.It has been proven that adipose-derived stem cells from young mice have better anti-aging effects.
