Changes of Gut Microbiota in Fecal Samples of Mice With Primary Biliary Cholangitis Associated With Depression by Using 16S rRNA Gene Sequencing Technology
10.3969/j.issn.1008-7125.2024.02.004
- VernacularTitle:基于16S rRNA基因测序技术分析原发性胆汁性胆管炎伴抑郁小鼠粪便样本中肠道菌群的变化
- Author:
Li TAO
1
;
Yongqing REN
;
Ziyang ZHANG
;
Jing WANG
Author Information
1. 内蒙古科技大学包头医学院研究生院(014040)
- Keywords:
Primary Biliary Cholangitis;
Cholestasis;
Depression;
16S rRNA Gene Sequencing Technology;
Intestinal Microbiota
- From:
Chinese Journal of Gastroenterology
2024;29(2):86-94
- CountryChina
- Language:Chinese
-
Abstract:
Background:There is a link between dysbiosis or disorder of gut microbiota and brain and liver diseases,and gut microbiota may affect the occurrence and development of primary biliary cholangitis(PBC)and depression through the gut-liver-brain axis.Aims:To analyze the gut microbiota in PBC mice associated with depression by using 16S rRNA gene sequencing technology.Methods:Twelve female mice were randomly divided into control group,cholestasis group,cholestasis+depression group and treatment group.Mice in the control group were given normal feed and water.Mice in the cholestasis group were fed with a feed containing 0.1%DDC for 2 weeks to establish cholestasis model.Mice in the cholestasis+depression group were stimulated with chronic mild unpredictable stress for 2 weeks,followed by 2 weeks of 0.1%DDC-containing feed to establish cholestasis+depression model.Mice in the treatment group were injected intraperitoneally with clomipramine hydrochloride(7.5 mg·kg-1·d-1)for 2 weeks based on the construction of cholestasis+depression model.During the modeling period,the behavioral changes of the mice were observed.After the modeling,the body weight was recorded,and the weight-bearing swimming test,forced swimming test,tail suspension test and HE staining of liver tissue were performed.The fresh feces were collected,and the changes of gut microbiota were analyzed by 16S rRNA gene sequencing technology.Results:Compared with the control group,the body weight of mice in cholestasis group and cholestasis+depression group was significantly decreased(P<0.05),and the depression-like behavior was significantly aggravated(P<0.05);compared with the cholestasis+depression group,the body weight of mice in treatment group was significantly increased(P<0.05),and the depression-like behavior was significantly ameliorated(P<0.05).Various degrees of cholestatic injury of the liver tissue were observed in the cholestasis group,cholestasis+depression group and treatment group.Compared with the cholestasis+depression group,the pathological injury of the liver tissue in treatment group was significantly reduced.After sequencing and flattening,5 491 operational taxonomic units(OTUs)were obtained in fecal samples.There were 162 common OTUs among the 4 groups.The microbial diversity and community composition of the control group,cholestasis group,cholestasis+depression group and treatment group were significantly different.At the phylum level,the abundance of Firmicutes in the cholestasis group was significantly increased;the abundance of Proteobacteria in the cholestasis+depression group was significantly increased;the abundance of both Firmicutes and Proteobacteria was significantly increased in the treatment group,while the abundance of Bacteroidetes was significantly decreased.LEfSe analysis was performed in species with significant differences among the groups,and the results showed that there were 38 microbial groups with LDA value>4.Conclusions:Significant changes are observed in gut microbiota composition and diversity in mice with PBC associated with depression.Antidepressant treatment can improve the abundance and diversity of gut microbiota,and regulating gut microbiota may become a new treatment strategy for PBC associated with depression.
