The Detection of Trypsin and Pepsin in middle Ear Effusion for Children with Acute Suppurative Otitis Media
10.3969/j.issn.1006-7299.2024.03.010
- VernacularTitle:儿童急性化脓性中耳炎中耳脓液中胰蛋白酶和胃蛋白酶检测分析
- Author:
Jinqiang SUN
1
,
2
;
Zeqi ZHAO
;
Zhengzhong HAN
;
Dan JIN
;
Yudi SHAO
;
Hao WANG
;
Tingting TANG
;
Wei LI
Author Information
1. 徐州医科大学第一临床医学院(徐州 221004)
2. 睢宁县人民医院耳鼻咽喉头颈外科
- Keywords:
Children;
Acute suppurative otitis media;
Pepsin;
Trypsin;
Laryngopharyngeal reflux disease
- From:
Journal of Audiology and Speech Pathology
2024;32(3):241-244
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the presence of pepsin and trypsin in the middle ear effusion of children with acute suppurative otitis media(ASOM).Methods Middle ear effusion samples were collected from 71 children with ASOM at Children's Hospital of Xuzhou.According to the characteristics of the middle ear effusions,the effu-sion was divided into serous and mucous types.The pH testing,Western Blotting(WB),and enzyme-linked immu-nosorbent assay(ELISA)were performed.Results ① There were 49.29％(35/71)of ASOM patients had a posi-tive RSI score(＞13).② The positive rate of pepsin in ASOM children was 49.29％(35/71),and the positive rate of trypsin was 42.25％(30/71).In addition,the positive rate of pepsin in RSI-positive children was 100％(35/35),and the positive rate of trypsin was 60％(21/35).There was no significant difference in the positive rate of pepsin and trypsin between serous and mucous middle ear effusion(P＞0.05).③ The pepsin concentration was 47.80(39.80,69.30)ng/ml and the trypsin concentration was 291.87±20.45 ng/ml in middle ear effusion of chil-dren with ASOM who had a positive WB test,and the trypsin concentration was significantly higher than pepsin(P＜0.05).There was no significant difference between the pepsin and the trypsin concentrations in serous and mu-cous middle ear effusion(P＞0.05).④ The pH value of mucous middle ear effusion was 7.39±0.28,and the pH value of serous middle ear effusion was 7.36±0.26.There was no significant difference between the pH value in se-rous and mucous middle ear effusion(P＞0.05).Conclusion The detection rates of pepsin and trypsin in middle ear effusion of children with ASOM were high which has important diagnostic value for children with ASOM combined with LPRD.
