Promoting effect of circulating FGF23 on atrial fibrosis in chronic kidney disease
- VernacularTitle:慢性肾脏病循环中FGF23对心房纤维化的促进作用
- Author:
Pan GAO
1
;
Bingxin XIE
;
Zandong ZHOU
;
Tong LIU
Author Information
- Keywords: kidney diseases; atrial fibrillation; fibrosis; atrial remodeling; fibroblast growth factors; receptors,fibroblast growth factor
- From: Tianjin Medical Journal 2024;52(9):917-923
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the possible mechanisms by which fibroblast growth factor(FGF)23 promoted atrial fibrosis in circulation of chronic kidney disease(CKD)by binding to atrial tissue fibroblast growth factor receptor(FGFR)4.Methods Twenty-two healthy male Sprague-Dawley(SD)rats were selected.Rats were randomly selected to undergo 5/6 nephrectomy and fed for 15 weeks to establish a CKD model(n=14).The remaining 8 rats were used as the sham group.The sham group(n=8)underwent the same surgery without removing renal tissue.Body weight,blood pressure,renal function,cardiac ultrasound,epicardial electrocardiography and pathological indices were monitored in both groups.Enzyme-linked immunosorbent assay(ELISA)method was used to determine the circulating levels of FGF23 in the two groups of rats.Transcriptomic analysis of left atrial tissue was performed to search for differentially expressed genes.Rat atrial fibroblasts were divided into the control group,the FGFR inhibitor group,the transforming growth factor-β(TGF-β)group and the TGF-β+FGFR inhibitor group.The expression levels of α-smooth muscle actin(α-SMA),collagenⅠ(Col Ⅰ)and phosphorylated protein kinase B(p-AKT)protein were detected by Western blot assay.Results Systolic blood pressure,blood urea nitrogen and creatinine were elevated in the CKD group of rats.Cardiac electrophysiological study showed that CKD could promote the occurrence of atrial fibrillation(AF)and atrioventricular block.Cardiac ultrasound suggested that the internal diameter of the left atrium was significantly increased in rats of the CKD group.Pathological findings showed that the left atrium in the CKD group underwent significant fibrosis,and epicardial electrical markers showed that left atrial electrical conduction velocity was significantly slower and conduction heterogeneity was significantly increased in the CKD group.These changes were accompanied by higher circulating FGF23.Western blot results showed that FGFR4 expression was upregulated in the CKD group.After blocking the FGF23/FGFR4 signaling pathway in atrial fibroblasts,the fibrosis-related proteins α-SMA,Col Ⅰ and p-AKT/AKT were decreased.Conclusion CKD promotes the occurrence of AF by inducing both structural and electrical remodeling.Increased circulating FGF23 promotes atrial fibrosis by activating the downstream AKT pathway binding to FGFR4 in atrial tissue.
