Effect of tRF-1:30 on the expression of inflammatory factors in renal tubular epithelial cells induced by high glucose
- VernacularTitle:tRF-1:30对高糖诱导的肾小管上皮细胞炎性因子表达的影响
- Author:
Yuwei XIA
1
;
Yunyang QIAO
;
Xuewei LIU
;
Huimin SHI
;
Gaoting QU
;
Aiqing ZHANG
;
Weihua GAN
Author Information
- Keywords: diabetic nephropathies; telomeric repeat binding protein 1; tRF-1:30-Gln-CTG-4; renal tubular epithelial cells; inflammatory factors; IKAROS family zinc finger protein 2
- From: Tianjin Medical Journal 2024;52(6):561-566
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effect and molecular mechanism of tRF-1:30-Gln-CTG-4(tRF-1:30)on the expression of inflammatory factors in high glucose(HG)-induced renal tubular epithelial cells(RTECs).Methods RTECs were divided into the control group,the HG group,the HG+tRF-1:30 mimic group,the HG+tRF-1:30 negative control(NC)group,the HG+si-IKZF2 group and the HG+si-NC group.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression levels of tRF-1:30,tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),monocyte chemoattractant protein-1(MCP-1)and IKAROS family zinc finger protein 2(IKZF2).Enzyme-linked immunosorbent assay(ELISA)was used to detect levels of TNF-α,IL-6 and MCP-1.Protein expression of IKZF2 was detected by Western blot assay.Dual-luciferase reporter assay was used to detect the targeting relationship between tRF-1:30 and IKZF2.Results The expression levels of inflammatory factors were elevated in HG-induced RTECs,and the expression level of tRF-1:30 was decreased(P＜0.05).Overexpression of tRF-1:30 significantly decreased expression levels of inflammatory factors in HG-induced RTECs(P＜0.05),and the expression level of IKZF2 was significantly increased(P＜0.05).Further knockdown of IKZF2 can inhibit the release of inflammatory factors,and the expression level of IKZF2 was down-regulated after overexpression of tRF-1:30.Double luciferase reporting experiment further verified the possible targeting relationship between tRF-1:30 and IKZF2.Conclusion Overexpression of tRF-1:30 inhibits the expression of inflammatory factors in HG-induced RTECs by target binding and negatively regulating the expression of IKZF2.
