miR-29b Regulates Vascular Smooth Muscle Cells Proliferation and Contractile Markers by Targeting KLF4
10.3870/j.issn.1672-0741.23.08.011
- VernacularTitle:miR-29b通过KLF4调控血管平滑肌细胞增殖及表型蛋白表达
- Author:
Junyan CAI
1
;
Didi ZHU
Author Information
1. 东南大学附属中大医院心内科,南京 210009
- Keywords:
miR-29b;
KLF4;
vascular smooth muscle cell;
ox-LDL
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2024;53(5):615-620
- CountryChina
- Language:Chinese
-
Abstract:
Objective The present study aimed to explore the effect of miR-29b on ox-LDL-stimulated vascular smooth muscle cells and the underlying mechanism.Methods VSMCs were divided into four groups:control+NC group,control+miR-29b group,ox-LDL+NC group and ox-LDL+miR-29b group.The cells were treated with 80 mg/L ox-LDL for 24 h to estab-lish the model.A cell counting Kit-8(CCK-8)assay was performed to evaluate VSMC proliferation.The expression of miR-29b,MCP-1,MCP-3,SM α-actin,SM MHC and KLF4 was detected via quantitative reverse-transcriptase polymerase chain reaction(qRT-PCR).The expression of MCP-1 and MCP-3 in the supernatant was detected via ELISA.Protein expression was exam-ined via Western blotting.A luciferase reporter assay was used to identify the relationship between KLF4 and miR-29b.Results We found that miR-29b was downregulated significantly in ox-LDL-stimulated VSMCs.The miR-29b mimics inhibited the pro-liferation and expression of KLF4,MCP-1 and MCP-3 and increased the levels of SM α-actin and SM MHC in the VSMCs trea-ted with ox-LDL.miR-29b could bind to the 3'-untranslated region(3'UTR)of KLF4 mRNA and subsequently inhibit the activi-ty of luciferase.The overexpression of KLF4 abrogated the effects of the miR-29b mimics.Conclusion In summary,miR-29b is able to regulate the proliferation and contractile markers of VSMCs via directly regulating KLF4.
