Regulatory Mechanism of the P2Y1-SK3 Pathway on Gastrointestinal Motility in Functional Dyspepsia Rats with PDGFRα+Cells
10.3870/j.issn.1672-0741.23.12.022
- VernacularTitle:PDGFRα+细胞上P2Y1-SK3通路对功能性消化不良大鼠胃肠动力的调控机制
- Author:
Deqian YANG
1
,
2
;
Qi CHEN
;
Xiaoli PAN
Author Information
1. 湖北中医药大学针灸骨伤学院,武汉 430061
2. 武汉设计工程学院亚心护理学院,武汉 430205
- Keywords:
functional dyspepsia;
platelet-derived growth factor receptor-α-positive interstitial cells;
interstitial cells of Cajal;
P2Y1;
small conductance Ca2+-activated K+channels
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2024;53(5):599-607
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study investigated the role of P2Y1-small conductance Ca2+-activated K+channel(SK3)channels in platelet-derived growth factor receptor-α-positive(PDGFRα+)cells in gastrointestinal motility in functional dyspepsia(FD).Methods Thirty rats were divided into control,model,and inhibitor groups(n=10 each group).The model and inhibitor groups were used to establish FD rat model,and the inhibitor group received MRS2500 via caudal injection.Behavioral and gastrointes-tinal assessments were conducted at the end of the experiment.Gastrointestinal electrophysiology was recorded,and gastric an-trum samples were analyzed histopathologically.Western blotting and qPCR were used to measure PDGFRα,C-kit(a specificity indicator for interstitial cells of Cajal),P2Y1,and SK3 expression levels.Immunofluorescence staining was used to assess pro-tein colocalization,and calcium assay kits were used to evaluate Ca2+flux.Results Following model establishment,experimental rats presented reduced activity,weight gain,and food intake,accompanied by decreased gastrointestinal motility and decreased expression of PDGFRα,C-kit,P2Y1,and SK3 in the gastric antrum.After MRS2500 treatment,FD rats presented increased weight gain and food consumption,with improved gastrointestinal motility.The expression of PDGFRα,P2Y1,and SK3 contin-ued to decrease,whereas C-kit expression and Ca2+levels increased.Notably,there was no coexpression of PDGFRα with C-kit,but significant coexpression of P2Y1 and SK3 was observed.Conclusion Stressors such as dietary factors and emotional dis-tress may increase inhibitory neurotransmitter release in gastrointestinal tissues,leading to reduced Ca2+sensitivity of the SK3 channel in PDGFRα+cells,affecting gastrointestinal motility in FD.
