Application effect of different peritoneal dialysis fluid collection methods for pathogenic bacteria culture in peritonitis dialysis-associated peritonitis
10.3760/cma.j.issn.1674-2907.2018.10.016
- VernacularTitle:不同腹膜透析液采集法在腹膜透析相关性腹膜炎致病菌培养中的应用效果
- Author:
Miaojuan XU
1
;
Qianwen ZHU
;
Kerui WANG
Author Information
1. 310006,树兰(杭州)医院(浙江大学国际医院)肾内科
- Keywords:
Peritoneal dialysis;
Peritonitis;
Pathogenic bacteria;
Bacterial culture;
Positive rate
- From:
Chinese Journal of Modern Nursing
2018;24(10):1195-1198
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate three different peritoneal dialysis fluid collection methods for pathogenic bacteria culture in peritonitis dialysis-associated peritonitis (PDAP), and provide clinical basis for the use of simple and effective peritoneal dialysis fluid collection method in primary hospital. Methods A total of 32 cases of PDAP patients treated in Shulan (Hangzhou) Hospital from June 2016 to July 2017 were included in the study using convenient sampling method. A number of 43 peritoneal dialysis fluid specimens were collected respectively using the traditional method, modified method and centrifugal method, and then were cultured for bacterial identification. Positive rate of bacteria, distribution of bacterial pathogens and reported time were compared among three groups. Results The positive rate of pathogenic bacteria showed that traditional method was 53.5%, modified method was 76.7% and centrifugal method was 83.7%. Compared with the traditional method, the positive rate of modified method and centrifugal method increased significantly (P<0.05). However, there was no statistical difference between the modified method and centrifugal method (P> 0.05). Three main species were epidermis staphylococcus, escherichia coli and staphylococcus aureus in three collection methods. In the modified method and centrifugal method, the positive rate of gram negative bacteria were higher than the traditional method (P<0.05); but there was no obvious difference on the positive rate of gram positive bacteria culture (P>0.05). The average reporting time of traditional culture was longer than the modified method and centrifugal method (P< 0.05). Conclusions Compared with traditional collection method, both modified method and centrifugal method could increase positive rate of bacteria culture in PDAP patients, especially gram negative bacteria, and had the advantage of rapid detection, which could provide clinical guidance for early treatment of peritonitis. Compared with centrifugal method, the modified method is simple, and worthy of promoting in primary hospital.
