Lycium Barbarum Polysaccharide Inhibits the Malignant Progression of Gallbladder Cancer Cells by Promoting Cellular Ferroptosis
10.3870/j.issn.1672-0741.23.11.001
- VernacularTitle:枸杞多糖通过促进胆囊癌细胞铁死亡抑制其恶性进展
- Author:
Guojun XIN
1
;
Jiancheng WANG
;
Yong YANG
Author Information
1. 宁夏回族自治区人民医院(宁夏医科大学附属自治区人民医院)肝胆外科,银川 750002
- Keywords:
lycium barbarum polysaccharide;
ferroptosis;
gallbladder cancer;
proliferation;
invasion;
GPX4
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2024;53(4):452-457
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of lycium barbarum polysaccharide(LBP)on the proliferation and metastasis of gallbladder cancer cells(NOZ and SGC-996)by regulating cellular ferroptosis.Methods NOZ and SGC-996 cells were cul-tured in vitro by treatment with different doses of LBP.The cells were divided into control group,20 μmol/L LBP group,40μmol/L LBP group,60 μmol/L LBP group,80 μmol/L LBP group,and LBP+Fe-1 groups.Cell activity was detected by MTT assay.Cell proliferation was detected by EdU assay.Cell invasion ability was detected by Transwell assay.Fe2+level was detec-ted by colorimetric assay,reactive oxygen species(ROS)and malondialdehyde(MDA)levels were determined by BODIPYT 581/591 C11 molecular probe.Western blot was used to detect glutathione peroxidase 4(GPX4),acyl-CoA Synthetase long-chain family member 4(ACSL4),β-catenin,and Wnt3A protein expression.Results Compared with the control group,cell viability,proliferation,and invasion were significantly decreased in the 40 μmol/L LBP,and 60 μmol/L LBP groups(all P＜0.05),and Fe2+level,ROS,and MDA activities were significantly increased(all P＜0.05),and GPX4,β-catenin,and Wnt3A protein ex-pressions were significantly reduced,and ACSL4 protein expression was significantly increased(all P＜0.05).Compared with the LBP group,cell viability,proliferation,and invasion ability were significantly increased in the LBP+Fer-1 group(all P＜0.05).Conclusion LBP inhibits the proliferation and metastasis of gallbladder cancer cells by inducing ferroptosis.
