Loganin regulates glycolipid metabolism by influencing intestinal microbiota and AMPK signaling in obese mice
- Author:
Xu BINGRUI
1
;
Ye ZIMENGWEI
;
Tian TIAN
;
Zhu RUYUAN
;
Liu CHENYUE
;
Fang XIN
;
Zhang DONGWEI
;
Fu MIN
;
Gao SIHUA
;
Zhao DANDAN
Author Information
1. School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing,102488,China
- Keywords:
Loganin;
Insulin resistance;
Obesity;
Glucose metabolism;
Lipid metabolism;
Gut microbiota;
AMPK signaling Pathway;
Mechanisms
- From:
Journal of Traditional Chinese Medical Sciences
2022;9(3):321-329
- CountryChina
- Language:Chinese
-
Abstract:
Objective:We aimed to observe the effects of loganin(Log)on serum glycolipid levels and probe the mechanisms focusing on intestinal flora and AMP-activated protein kinase(AMPK)signaling in obese mice.Methods:A high-fat diet was given for 12 consecutive weeks to generate the obesity model in institute of cancer research(ICR)mice.Body weight was measured weekly and fasting blood glucose(FBG)was determined every 2 weeks.Both the oral glucose tolerance test and the intraperitoneal insulin tolerance test were performed.The serum levels of total cholesterol(TC),triglyceride,high-density lipoprotein-cholesterol,low-density lipoprotein-cholesterol(LDL-C),and free fatty acids(FFA)were measured.The expression of key proteins in the AMPK signaling pathway in skeletal muscle tissue was detected by immunoblotting,and gut microbiota were characterized using 16S rDNA sequencing.Results:Log significantly decreased the body weight and the FBG in obese mice(P＜.05),and it could restore FBG to normal levels.The total cholesterol,LDL-C,and FFA levels were significantly reduced by Log compared with the obese controls(TC:P=.0020;LDL-C:P=.0233;FFA:P=.0127),and the glucose tolerance of animals was significantly improved(P=.0477).The western blot results showed that Log could upregulate the protein expression of Adenosine 5'-monophosphate(AMP)-activated protein kinase(AMPKα),Sirtuin 1(SIRT1),and peroxisome proliferator-activated receptor-gamma coactivator-1 alpha(PGC1α)in skeletal muscle tissue of obese mice.16S rDNA sequencing indicated that Log reduced the diversity of the gut flora in feces and altered the floral composition of obese mice.Conclusions:Log was effective in reducing body weight and improving glucolipid metabolism in obese mice,probably through activating AMPK signaling and regulating intestinal microbial diversity.
