Effect of pediatric tuina on hypothalamic metabolites in young rabbits using liquid chromatography-mass spectrometry
- Author:
Liu ZHIFENG
1
;
Jiao YI
;
Yu TIANYUAN
;
Zhang YINGQI
;
Liu DI
;
Wang HOURONG
;
Xu YAJING
;
Guan QIAN
;
Lv TAOTAO
;
Shu JIAN
Author Information
1. School of Acupuncture-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing,102488,China
- Keywords:
Fever;
Pediatric tuina;
Metabolomics;
Liquid chromatography coupled with mass spectrometry;
Hypothalamus;
Interleukin-1β;
Tumor necrosis factor-α;
Prostaglandin E2
- From:
Journal of Traditional Chinese Medical Sciences
2022;9(2):188-196
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the changes in the hypothalamic metabolites of lipopolysaccharide(LPS)febrile young rabbits after the treatment with pediatric tuina.Methods:A total of 30 young rabbits were randomly assigned into three groups:the normal group,the model group,and the tuina group.Both the model group and the tuina group were injected intrave-nously with LPS."Six antipyretic manipulations"(pushing Tianmen,pushing Kangong,kneading Taiyang,kneading Erhougaogu,clearing Tianheshui,and pushing Jizhu)were administered 1 h after the LPS in-jection in the tuina group.The rectal temperatures of the young rabbits were monitored during the experiment to explore the antipyretic effect.Three hours after the injection,the content of interleukin-1 β(IL-1β),tumor necrosis factor(TNF)-α,and prostaglandin E2(PGE2)in the serum was detected.In addition,liquid chromatography-mass spectrometry(LC-MS)was used for the hypothalamus metabolomics.Results:Compared with the model group,the rectal temperature of the tuina group was decreased at 2 h and 3 h after the LPS injection(P=.04,P=.03,respectively),and the content of IL-1β,TNF-α,and PGE2 was decreased(P=.03,P=.003,and P=.008,respectively).The metabolomics results showed that there were 23 potential biomarkers after the tuina intervention,enriching 27 pathways.Lipid metabolites,especially glycerophospholipids,were a majority of the altered metabolites.The primary metabolic pathways affected by tuina included the arachidonic acid metabolism,the GABAergic synapse,D-gluta-mine and D-glutamate metabolism,and the glutamatergic synapse.Conclusion:Pediatric tuina reduced the temperature of the febrile rabbits and downregulated the expression of IL-1β,TNF-α,and PGE2,and the antipyretic mechanism may be related to changes in metabolites and metabolic pathways in the hypothalamus.
