Inhibitory effect of small-molecule compound AM679 targeting elongation-factor binding protein 2 on hepatitis B virus in vitro

VernacularTitle:靶向延伸因子结合蛋白?2?的小分子化合物AM679体外对乙型肝炎病毒的抑制作用
Author: Huijing FANG ¹ ; Jinyuan CAI ; Xiaoxue HOU ; Jiali SONG ; Linya PENG ; Chuanlong ZHU
Author Information

1. 南京医科大学第一附属医院感染病科，南京　210029
Keywords: Hepatitis B virus; EFTUD2; Small molecule compound; AM679
From: Chinese Journal of Hepatology 2024;32(4):318-324
CountryChina
Language:Chinese
Abstract: Objective:To explore the antiviral activity of the small-molecule compound AM679 in hepatitis B virus (HBV) replication and infection cell models.Methods:The positive regulatory effect of AM679 on EFTUD2 expression was validated by qPCR and Western blotting. HepAD38 and HepG2-NTCP cells were treated with AM679 (0.5, 1, and 2 nmol/L). Negative control, positive control, and AM679 combined with the entecavir group were set up. HBV DNA intra-and extracellularly, as well as the expression levels of intracellular HBV total RNAs and 3.5kb-RNA changes, were detected with qPCR. Hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) levels were measured in the cell supernatant by an enzyme-linked immunosorbent assay (ELISA). The t-test method was used for the statistical analysis of the mean difference between groups.Results:EFTUD2 mRNA and protein expression levels were significantly increased in HepAD38 and HepG2-NTCP cells following AM679 treatment, with a statistically significant difference ( P ?