The correlation between microRNA let-7a-3 methylation and IGF-Ⅱ expression in esophageal squamous cell carcinoma
10.11904/j.issn.1002-3070.2024.03.005
- VernacularTitle:食管鳞癌中microRNA let-7a-3甲基化与IGF-Ⅱ的相关性
- Author:
Gongjian ZHU
1
;
Hongyun GUO
;
Yue BAI
;
Xiaomin WANG
;
Xiaokang ZHU
;
Dongyan LIU
;
Yuqin LIU
Author Information
1. 甘肃省医学科学研究院/中山大学附属肿瘤医院甘肃医院科教科(兰州 730050)
- Keywords:
Esophageal squamous cell carcinoma;
let-7a-3;
Methylation;
Insulin like growth factor 2
- From:
Practical Oncology Journal
2024;38(3):173-178
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to explore the correlation between the methylation status of microRNA let-7a-3 in esophageal squamous cell carcinoma(ESCC)and the expression of insulin-like growth factor 2(IGF-Ⅱ).Methods The methylation specific PCR(qMSP)was used to detect the methylation status of let-7a-3 in 83 cases of esophageal cancer and corre-sponding adjacent normal tissues.The enzyme linked immunosorbent assay(ELISA)was used to detect the expression of IGF-Ⅱ in plasma.Results The degree of let-7a-3 methylation in cancer tissues of 83 patients with ESCC was significantly higher than that in normal tissues adjacent to cancer(P<0.001).The expression of IGF-Ⅱ in the plasma of 83 patients with ESCC was positively corre-lated with the methylation degree of let-7a-3,which was statistically significant(r=0.600,P<0.001).Conclusion microRNAlet-7a-3 may participate in the occurrence and progression of ESCC by regulating the methylation of downstream molecules,which is of great significance for understanding the mechanisms of ESCC development and providing a basis for the diagnosis and prognosis of ESCC.
