Value of constructing a non-invasive diagnostic model based on serum heme oxygenase-1 and glucose regulatory protein 78 for non-alcoholic fatty liver disease
10.3760/cma.j.cn501113-20230830-00079
- VernacularTitle:基于血清血红素加氧酶-1和葡萄糖调节蛋白78构建的无创诊断模型对非酒精性脂肪性肝病的价值
- Author:
Jiacen CAO
1
;
Hongkun ZHANG
;
Chuanmiao LIU
;
Shousong ZHAO
;
Yuemin NAN
;
Dongdong LI
Author Information
1. 蚌埠医学院第一附属医院感染科，蚌埠 233030
- Keywords:
Fatty liver;
Diagnosis;
Heme oxygenase-1;
Glucose-regulated protein 78
- From:
Chinese Journal of Hepatology
2024;32(3):228-234
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the clinical application value of serum heme oxygenase (HO)-1expression level in non-alcoholic fatty liver disease (NAFLD) and, based on that, establish a diagnostic model combined with glucose regulatory protein 78 (GRP78) so as to clarify its diagnostic effectiveness and application value.Methods:A total of 210 NAFLD patients diagnosed by abdominal B-ultrasound and liver elastography were included, and at the same time, 170 healthy controls were enrolled. The general clinical data, peripheral blood cell counts, and biochemical indicators of the research subjects were collected. The expression levels of HO-1 and GRP78 were detected using an enzyme-linked immunosorbent assay. Multivariate analysis was used to screen independent risk factors for NAFLD. Visual output was performed through nomogram diagrams, and the diagnostic model was constructed. Receiver operating characteristic curve (ROC), calibration curve, and decision curve analysis (DCA) were used to evaluate the diagnostic effectiveness of NAFLD. Measurement data were analyzed using a t-test or Mann-Whitney U rank sum test to detect data differences between groups. Enumeration data were analyzed using the Fisher's exact probability test or the Pearson χ2 test. Results:Compared with the healthy control group, the white blood cell count, aspartate aminotransferase (AST), alanine aminotransferase, gamma-glutamyl transferase (GTT), fasting blood glucose (Glu), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), serum HO-1, and GRP78 levels were significantly increased in the NAFLD group patients ( P ?
