Overexpression of tuftelin and KLF-5 and its clinicopathological features in hepatitis B virus-related hepatocellular carcinoma

VernacularTitle:乙型肝炎病毒相关肝癌釉丛蛋白和KLF-5过表达及其临床病理特征
Author: Junling YANG ¹ ; Rongfei FANG ; Qun XIE ; Bojun TAI ; Dengfu YAO ; Min YAO
Author Information

1. 南通大学附属医院临床医学研究中心和医学院免疫学系，南通　226001
Keywords: Hepatocellular carcinoma; Hepatitis B virus; TUFT1; KLF5; Clinicopathological features; Prognosis
From: Chinese Journal of Hepatology 2024;32(2):148-154
CountryChina
Language:Chinese
Abstract: Objective:To analyze and evaluate the expressions and clinical value of tuftelin (TUFT1) and Krüppel-like factor 5 (KLF5) in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) tissues.Method:KLF5 mRNA and TUFT1 mRNA transcriptional status in cancer and non-cancer groups were compared according to the Cancer Genome Atlas (TCGA) database. The differences and prognostic value between the groups were analyzed. Postoperative liver cancer and its paired pericancerous tissues, with the approval of the ethics committee, were collected to build tissue chips. The expression of KLF5 and TUFT1 and their intracellular localization were verified by immunohistochemistry. Tissue expression and clinicopathological characteristics were analyzed by immunoblotting. SPSS software was used to analyze the relationship between SPSS and patient prognosis.Results:The transcription level of TUFT1 or KLF5 mRNA was significantly higher in the HCC group than the non-cancer group ( P ?