The effects and mechanism of long non-coding RNA CASC15 on proliferation,invasion and migration of hepatocellular car-cinoma
10.11904/j.issn.1002-3070.2024.01.004
- VernacularTitle:长链非编码RNA CASC15对肝细胞癌增殖、侵袭和迁移的影响及作用机制
- Author:
Dongyang WU
1
;
Qingshan CAI
;
Dong LIU
;
Ganggang ZUO
;
Shudong LI
;
Liyou LIU
;
Jianxing ZHENG
Author Information
1. 唐山中心医院肝胆外科(唐山 063000)
- Keywords:
Cancer susceptibility gene 15;
miR-144-3p;
Leucine-rich repeative sequence protein 1;
Hepatocellular carcino-ma cells;
Cell biological function
- From:
Practical Oncology Journal
2024;38(1):18-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to investigate the molecular regulatory mechanism of cancer susceptibility candidate 15(CASC15),a long-stranded non-coding RNA(lncRNA),in hepatocellular carcinoma(HCC).Methods Bioinformat-ics methods were used to predict the expression of target genes and analyze the relationship between the expression of target genes and the survival time of patients;Hepatocellular carcinoma tissues and adjacent tissues from patients with HCC were collected;CCK-8,Tr-answell,and flow cytometry experiments were used to detect proliferation,invasion,migration and apoptosis of SMMC7721 cells and Huh-7 cells;The dual-luciferase assay was used to detect the targeting relationship between miR-144-3p and CASC15,as well as leucine rich repeat containing protein 1(LRRC1);RT-qPCR and Western blot were used to detect mRNA and protein expression of target genes;Immunofluorescence was used for protein localization of target genes;Replicate experiment was performed to verify the effect of CASC15/miR-144-3p/LRRC1 on the progression of HCC.In vivo experiment was performed to verify the effect of CASC15 on HCC progression.Results TCGA database and RT-qPCR assay showed high expression of CASC15,low expression of miR-144-3p,and high expression of LRRC1 in HCC tissues and cells(P<0.05).The results of cell function experiments on proliferation,inva-sion and migration showed that CASC15 and LRRC1 played a promoting role in tumor development,while miR-144-3p had an inhibi-tory effect,consistent with the results of apoptosis experiments(P<0.05).Cell function experiments showed that CASC15 inhibited miR-144-3p function,miR-144-3p inhibited LRRC1,and CASC15 bound to miR-144-3p,leading to the upregulation of LRRC1.The replicate experimental results indicated that CASC15 promoted LRRC1 expression through inhibiting miR-144-3p,thereby pro-moting HCC cell proliferation,invasion and migration,and inhibiting apoptosis.Conclusion CASC15 may promote HCC progression by regulating the miR-144-3p/LRRC1 axis.
