UGT1A1 gene mutation spectrum with indirect hyperbilirubinemia in children
10.3760/cma.j.cn501113-20230115-00030.
- VernacularTitle:儿童高间接胆红素血症的 UGT1A1基因突变谱
- Author:
Ying SHEN
1
;
Hongmei GUO
;
Yucan ZHENG
;
Bixia ZHENG
;
Kunlong YAN
;
Guiping KONG
;
Qian LIN
;
Yu JIN
;
Zhifeng LIU
;
Mei LI
Author Information
1. 南京医科大学附属儿童医院消化科, 南京 210008
- Keywords:
Indirect hyperbilirubinemia;
Diagnose;
Genetic mutation;
Etiological analysis;
UDP-glucuronyl transferase 1A1
- From:
Chinese Journal of Hepatology
2024;32(2):119-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the relevancy between the uridine diphosphate-glucuronylgly-cosyltransferase 1A1 (UGT1A1) gene mutation and the phenotype of indirect hyperbilirubinemia in children.Methods:Sixteen cases with indirect hyperbilirubinemia who visited the Department of Gastroenterology, Children's Hospital of Nanjing Medical University from July 2013 to November 2019 were retrospectively analyzed and were divided into Gilbert syndrome (GS), Crigler-Najjar syndrome type II (CNS-II), and indirect hyperbilirubinemia groups unexplained by UGT1A1 gene mutations. The differences in gene mutation site information and general clinical data were compared. The association between gene mutation spectrum and bilirubin level was explored by t-test analysis.Results:Ten of the sixteen cases with indirect hyperbilirubinemia had GS, three had CNS-II, and three had indirect hyperbilirubinemia unexplained by UGT1A1 gene mutations. A total of six mutation types were detected, of which c.211G?>?A accounted for 37.5% (6/16), c.1456T?>?G accounted for 62.5% (10/16), and TATA accounted for 37.5% (6/16), respectively. Compared with the GS group, the CNS group had early disease onset incidence, high serum total bilirubin ( t ?=?5.539, P ??0.05) and age of onset ( t ?=?0.3611, P ?>?0.05) between the two groups. There was no significant correlation between the number of UGT1A1 gene mutations and serum bilirubin levels. Children with c.1456T?>?G homozygous mutations had the highest serum bilirubin levels. Conclusion:The common pathogenic variants of the UGT1A1 gene sequence are c.1456T?>?G, c.211G?>?A, and TATA, indicating that these site mutations are related to the occurrence of indirect hyperbilirubinemia and have important guiding significance for the etiological analysis of indirect hyperbilirubinemia in children.
