The mechanism of cyclin D1 ameliorates renal ischemia-reperfusion-induced acute kidney injury by pro-motingglycolysis
10.3969/j.issn.1006-5725.2024.21.009
- VernacularTitle:Cyclin D1通过促进糖酵解改善肾脏缺血再灌注诱导的急性肾损伤的机制
- Author:
Yuliang HUANG
1
;
Ying TANG
;
Wenjuan YU
;
Junzhe CHEN
Author Information
1. 南方医科大学第三附属医院肾内科(广东 广州 510630)
- Keywords:
renal ischemia-reperfusion injury;
acute kidney injury;
tubular epithelial cells;
Cyclin D1;
glycolysis;
energy metabolism
- From:
The Journal of Practical Medicine
2024;40(21):3013-3022
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of CCND1 on renal ischemia-reperfusion-induced acute kidney injury through the promotion of glycolysis and elucidate its underlying molecular mechanism,thereby offering a novel therapeutic target for acute kidney injury.Methods We selected 8-week-old male C57BL/6 mice to establish a model of renal ischemia-reperfusion injury(IRI).To investigate the role of CCND1 in acute kidney injury(AKI),we employed a CCND1 overexpression plasmid and a CCND1 interference plasmid for both in vivo and in vitro experi-ments.Kidney function was evaluated using creatinine and urea nitrogen test kits,while glycolysis and indicators of renal tubule epithelial cell damage were assessed through quantitative real-time PCR,Western blotting,immunohis-tochemistry,and immunofluorescence techniques.Results In the model of renal ischemia-reperfusion-induced acute renal injury,down-regulation of CCND1 expression in renal tubular epithelial cells resulted in cellular and tissue damage.However,when an overexpression plasmid for CCND1 was administered in vivo,it significantly improved kidney function and reduced kidney injury in IRI mice.The overexpression of CCND1 promoted glycolysis,pyruvate production,and increased energy production.We further confirmed the role of CCND1 in vitro where its overexpres-sion promoted glycolysis,enhanced energy production,and alleviated AKI.Conversely,knockdown of CCND1 inhibited glycolysis leading to severe impairment in cell energy production and exacerbation of injury.Conclusions In summary,down-regulation of CCND1 expression in renal tubular epithelial cells is observed in acute kidney injury,while overexpression of CCND1 can ameliorate acute kidney injury induced by renal ischemia-reperfusion.This mechanism may be attributed to the promotion of glycolysis and timely restoration of energy supply to cells and tissues facilitated by CCND1 overexpression.
