Sophora davidii Hance leaves total alkaloids Attenuate Lipopolysaccharide-induced inflammatory response in RAW264.7 cell by Inhibiting the MAPK/NF-κB signaling pathway
10.3969/j.issn.1006-5725.2024.20.004
- VernacularTitle:白刺花叶总生物碱通过抑制MAPK/NF-κB信号通路减轻脂多糖诱导的RAW264.7细胞炎症反应
- Author:
Shengnan JIANG
1
;
Wenbing ZHI
;
Jing CHEN
;
Tingting SUN
;
Zongren XU
;
Shuai LIU
;
Hong ZHANG
;
Ye LI
;
Yang LIU
Author Information
1. 陕西省中医药研究院(陕西省中医医院)(陕西西安 710003)
- Keywords:
Sophora davidii(Franch.)Skeels;
total alkaloid;
anti-inflammation;
action mechanism
- From:
The Journal of Practical Medicine
2024;40(20):2835-2840
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the in vitro anti-inflammatory effects of Sophora davidii Hance leaves total alkaloids(SDLTAs)and possible molecular mechanisms.Methods The lipopolysaccharide(LPS)-induced inflammation model of RAW264.7 cells was used,and different concentrations of SDLTAs(50,100 and 200 μg/mL)were administered,and the effect of SDLTAs on cellular NO expression was detected by the Griess method;ELISA method was used to detect the effect of SDLTAs on the expression of IL-6,TNF-α and IL-1β;The expression of iNOS,NF-κB p65 and IκBα mRNA was detected by RT-qPCR;Western blotting was used to detecte the expres-sion of p-p38,p-p65 and p-JNK in the cells and NF-κB p65 in the nucleus.Results SDLTAs could significantly inhibit the LPS-induced inflammatory response in RAW264.7 cells.SDLTAs significantly decreased the secretion of NO,IL-6,TNF-α and IL-1β in cells(P<0.01),and significantly decreased the mRNA expressions of iNOS,NF-κB p65 and IκBα in cells(P<0.01).Significantly decreased the protein expression of p-p38,p-p65 and p-JNK in cells and NF-κB p65 in nucleus(P<0.01).Conclusion SDLTAs can exert anti-inflammatory effects by regulating the MAPK/NF-κB signalling pathway.
