The effect of umbilical cord stem cell exosomes on the proliferation of dermal papilla cells
10.3969/j.issn.1006-5725.2024.20.003
- VernacularTitle:人脐带干细胞外泌体对人毛乳头细胞增殖的影响
- Author:
Qing LUO
1
;
Jinjin HUANG
;
Tingting REN
;
Ruihua ZHOU
;
Donghua XU
;
Zhenhua WANG
;
Guoying WANG
Author Information
1. 山东第二医科大学(山东潍坊 261053)
- Keywords:
dermal papilla cells;
umbilical cord mesenchymal stem cells;
exosomes;
prolifera-tion;
regeneration
- From:
The Journal of Practical Medicine
2024;40(20):2828-2834
- CountryChina
- Language:Chinese
-
Abstract:
Objective We tried to investigate the effects of human umbilical cord mesenchymal stem cell exosomes(hUCMSC-Exos)on the proliferation of human dermal papilla cells(HDPCs)and the mechanism of hUCMSC-Exos promoting hair growth.Methods HDPCs were isolated using two-step enzymatic method and cul-tured in vitro.Human umbilical cord mesenchymal stem cells(hUC-MSCs)were cultured.Cell culture supernatant was collected,and exosomes were isolated and extracted using high-speed centrifugation.Electron microscopy,particle size,and surface marker identification were performed on them.Dihydrotestosterone(DHT)induces HDPCs and establishment of an androgenic alopecia cell model.Co-culture hUCMSC-Exos with HDPCs,cell proliferation experiment(EdU)was used to detect the relative activity of induced HDPCs.Real-time qPCR was used to detect the expression level of alkaline phosphatase(ALP),and Western blot was used to detect β-catenin,Wnt10b,GSK-3β expression at the protein level.Results The obtained primary HDPCs,hUC-MSCs,and hUCMSC-Exos were all conformed to the characteristics of dermal papilla cells,mesenchymal stem cells,and exosomes.The num-ber of EdU positive cells significantly increased,and exosomes could effectively promote the proliferation of HDPCs(P<0.05),enhance the vitality of HDPCs and alleviate the damage caused by DHT(P<0.05).Real-time qPCR showed that exosomes could enhance the expression level of ALP gene(P<0.05)and hair follicle induction ability.Western Blot confirmation β-catenin,Wnt10b,GSK-3β were differences in expression at the protein level(P<0.05).Conclusions HUCMSC-Exos could promote DHT induced proliferation of HDPCs,enhance their hair follicle regeneration and repair ability,and its mechanism may be related to the activation of Wnt/β-catenin signaling pathway.
