Effects of agatroban combined with mitochondrial transplantation on endothelial function and hemorheol-ogy in mice with cerebral ischemia-reperfusion injury
10.3969/j.issn.1006-5725.2024.19.001
- VernacularTitle:阿加曲班联合线粒体移植对脑缺血再灌注损伤小鼠内皮功能及血流变学的影响
- Author:
Yingjun TAO
1
,
2
;
Tengzhu REN
;
Feng WEI
;
Xin-Tong LIU
Author Information
1. 南华大学衡阳医学院广东省第二人民医院研究生培养基地(湖南衡阳 421001)
2. 广东省第二人民医院神经内科(广东广州 510317)
- Keywords:
agatroban;
mitochondrial transplantation;
cerebral ischemia-reperfusion injury;
endo-thelial function;
hemorheology
- From:
The Journal of Practical Medicine
2024;40(19):2665-2671
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the effects of agatroban combined with mitochondrial transplantation on endothelial function and hemorheology in mice with cerebral ischemia-reperfusion injury.Methods 50 SPF grade Kunming mice,with 10 as the normal group,38 mices were successfully modeled and divided into a model group of 9,a mitochondrial transplantation group of 9,an Agatroban group of 10,and a combination group of 10.The mitochondrial transplantation group was given exogenous mitochondrial extract,the agatroban group was given agatroban,the combined group was given agatroban combined with mitochondrial transplantation,and the blank group and model group were injected with normal saline.Observation of changes in cerebral infarction volume,pathological morphology of brain tissue,endothelial function,hemorheology,and relative expression of apoptosis related proteins in mice after 7 days of intervention.Results Compared with normal group,cerebral infarction volume ratio,ET-1,whole blood viscosity,plasma viscosity,Caspase-3 and Bax protein expression levels were increased in model group,mitochondrial transplantation group,agatroban group and combined group,while NO level and Bcl-2 protein expression levels were decreased(P<0.05).Compared with the model group,the cerebral infarction volume ratio,ET-1,whole blood viscosity,plasma viscosity,Caspase-3 and Bax protein expressions in the mitochondrial transplantation group,agatroban group and combined group were decreased,and the combined group was lower than the mitochondrial transplantation group and agatroban group,and the NO level and Bcl-2 protein expression were increased.The combined group was higher than that of mitochondrial transplantation group and agatriban group(P<0.05).Conclusion In mice with cerebral ischemia-reperfusion injury,endothelial function injury was improved,hemorheology was regulated,and brain injury was alleviated after agatroban combined with mitochondrial transplantation.
