Ascites CD100 levels and immunomodulation effects in the peripheral blood of patients with liver cirrhosis combined with spontaneous bacterial peritonitis
10.3760/cma.j.cn501113-20220314-00108
- VernacularTitle:肝硬化合并自发性细菌性腹膜炎患者外周血和腹水CD100水平和免疫调节作用
- Author:
Huanrong HOU
1
;
Yi KANG
Author Information
1. 河南省人民医院感染性疾病科，郑州 450003
- Keywords:
Liver cirrhosis;
Peritonitis;
Ascites;
CD100;
T-lymphocytes
- From:
Chinese Journal of Hepatology
2023;31(2):138-146
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the level and detection of ascites CD100 on the activity of CD4 + and CD8 + T lymphocytes in vitro in the peripheral blood of patients with liver cirrhosis combined with spontaneous bacterial peritonitis. Methods:Peripheral blood and ascites were collected from 77 cases of liver cirrhosis (49 patients with liver cirrhosis combined with simple ascites and 28 patients with liver cirrhosis combined with SBP), and peripheral blood was collected from 22 controls. Soluble CD100 (sCD100) in peripheral blood and ascites was detected by an enzyme-linked immunosorbent assay. Flow cytometry was used to detect membrane-bound CD100 (mCD100) on the surface of CD4 + and CD8 +T lymphocytes. CD4 + and CD8 +T lymphocytes in ascites were sorted. CD4 +T lymphocyte proliferation, key transcription factor mRNA, and secreted cytokine changes, as well as CD8 +T lymphocyte proliferation, important toxic molecule mRNA, and secreted cytokine changes, were detected after CD100 stimulation. The killing activity of CD8 +T cells was detected by direct contact and indirect contact culture systems. Data conforming to normality were compared using one-way ANOVA, a student's t-test, or a paired t-test. Data that did not conform to a normal distribution were compared using either the Krusal-Willis test or the Mann-Whitney test. Results:There was no statistically significant difference in plasma sCD100 level between patients with liver cirrhosis combined simple ascites (1 415 ± 434.1) pg/ml, patients with liver cirrhosis combined with SBP (1 465 ± 386.8) pg/ml, and controls (1 355 ± 428.0) pg/ml ( P = 0.655). The ascites sCD100 level was lower in patients with liver cirrhosis combined with SBP than that of patients with simple ascites [(2 409 ± 743.0) pg/ml vs. (2825±664.2) pg/ml, P=0.014]. There was no statistically significant difference in the level of mCD100 in peripheral blood CD4 + and CD8 + T lymphocytes among the three groups ( P > 0.05). The levels of mCD100 in ascites CD4 + and CD8 + T lymphocytes were higher in patients with liver cirrhosis combined with SBP than those in patients with simple ascites ( P < 0.05). CD100 stimulation had no significant effect on the proliferation of CD4 + and CD8 +T lymphocytes in the ascites of patients with liver cirrhosis combined with SBP ( P > 0.05). There were no significant effects on the expression of transcription factors in effector CD4 +T lymphocytes (T-bet, retinoic acid associated solitary nuclear receptor γt, aromatic hydrocarbon receptor) or secretion of cytokines (interferon-γ, 17, and 22) ( P > 0.05). CD100 stimulation had increased the relative expression of perforin, granzyme B, and granlysin mRNA and the levels of secreted interferon-γ and tumor necrosis factor-α, killing activity in ascites CD8+ T lymphocytes of patients with liver cirrhosis combined with SBP ( P < 0.05). Conclusion:The active form of CD100 is sCD100 instead of mCD100. There is an imbalance between the expression of sCD100 and mCD100 in the ascites of patients with cirrhosis combined with SBP. sCD100 can enhance the function of CD8 +T lymphocytes in the ascites of patients with cirrhosis combined with SBP and thus is one of the potential therapeutic targets.
