Effects of miR-223 on prostate cancer cell damage by regulating Keap1/Nrf2/ARE signaling pathway
10.3969/j.issn.1006-5725.2024.17.004
- VernacularTitle:miR-223通过调控Keap1/Nrf2/ARE信号通路对前列腺癌细胞损伤的影响
- Author:
Zhishi WANG
1
;
Guiling LI
;
Jingguo CHEN
;
Hong WANG
Author Information
1. 海南省中医院泌尿外科(海口 570203)
- Keywords:
prostate cancer;
microRNA-223;
Kelch-like epichlorohydrin associated protein 1/nuclear factor E2 related factor 2/antioxidant response element;
cell injury
- From:
The Journal of Practical Medicine
2024;40(17):2375-2380
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of microRNA-223(miR-223)on prostate cancer cell damage by regulating the Kelch-like epichlorohydrin related protein 1(Keap1)/nuclear factor E2 related factor 2(Nrf2)/antioxidant response element(ARE)signaling pathway.Methods The prostate cancer cell line PC3 was cultured and randomly divided into control,down-regulated miR-223,and up-regulated miR-223 groups.Changes in miR-223 expression,cell proliferation rate,cell migration number,cell invasion number,apoptosis rate,and expression level of Keap1/Nrf2/ARE signaling pathway were explored.Results Compared with the control group,the cell invasion number,cell migration number,cell proliferation rate,Nrf2 and ARE expression increased at 24,48 and 72 h in down-regulated miR-223 group,while the expressions of miR-223,Keap1 and apoptosis rate decreased(P<0.05).Compared with the down-regulated miR-223 group,24,48 and 72 h cell proliferation rate,cell invasion number,cell migration number,ARE and Nrf2 expression decreased in the up-regulated miR-223 group,while miR-223,apoptosis rate and Keap1 expression increased(P<0.05).Conclusion Regulation of miR-223 effectively ameliorates prostate cell injury,and the mechanism may be related to the Keap1/Nrf2/ARE signaling pathway.
