Molecular mechanism of young Sca-1 bone marrow stem cell on old cardiac fibroblast cell apoptosis in aging mice
10.3969/j.issn.1006-5725.2024.17.003
- VernacularTitle:年轻干细胞抗原-1阳性骨髓干细胞调控年老小鼠心脏成纤维细胞凋亡的分子机制
- Author:
Rao LÜ
1
,
2
;
Jiadi YU
;
Liuzhen LI
;
Chulan ZHAN
;
Liyue ZHAO
;
Yueliang LI
;
Jun DONG
;
Jiao LI
Author Information
1. 广州医科大学附属清远医院(清远市人民医院),第六临床学院,广州医科大学心脏病学系(广东清远 511518)
2. 广州医科大学附属第二医院老年病科(广州 510260)
- Keywords:
stem cell;
cardiac fibroblast cell;
apoptosis;
cell survival;
growth factors;
Sca-1
- From:
The Journal of Practical Medicine
2024;40(17):2369-2374
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of Sca-1 bone marrow derived stem cells on apoptosis in murine cardiac fibroblasts and the molecular mechanisms of young(Y)Sca-1 bone marrow stem cell(BMSC)on old(O)cardiac fibroblast cell(CFC)apoptosis.Methods The apoptosis and survival of Y and O CFC were assessed under hypoxic conditions.Co-cultures of Y and O Sca-1 bone marrow-derived mesenchymal stem cells(BMSC)with O CFC were established to investigate the impact of Sca-1 BMSC on the apoptotic response and viability of O CFC,employing TUNEL staining,qRT-PCR,Western Blot,and CCK8 assays.Furthermore,differential secretion profiles of growth factors by Y and O Sca-1 BMSC were compared using qRT-PCR and ELISA analysis.Results Compared to Y CFC,O CFC exhibited an increased rate of apoptosis and a decreased rate of cell survival.However,when compared to O cells,Y Sca-1 BMC significantly reduced apoptosis in O CFC and enhanced cell survival.Moreover,Y Sca-1 BMSC demonstrated a higher secretion of GDF5(Growth Differentiation Factor 5)than O cells(P<0.05).Importantly,the protective effects of Y Sca-1 BMSC on apoptosis and survival in O CFC were abolished upon neutral-ization of GDF5 expression.Conclusion Y Sca-1 BMSC decreases O CFC apoptosis through GDF5.
