The effect of miR-143-3p on pyroptosis of ulcerative colitis cells by regulating TLR2/NF-κB/NLRP3
10.3969/j.issn.1006-5725.2024.15.003
- VernacularTitle:miR-143-3p靶向调控TLR2/NF-κB/NLRP3通路对溃疡性结肠炎细胞焦亡的影响
- Author:
Xiuli SHI
1
;
Jiaqi CHEN
;
Fan ZHU
;
Juan ZENG
;
Na WU
Author Information
1. 江西中医药大学(南昌 330004)
- Keywords:
ulcerative colitis;
cell pyroptosis;
miR-143-3p;
TLR2;
TLR2/NF-κB/NLRP3
- From:
The Journal of Practical Medicine
2024;40(15):2056-2062
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of miR-143-3p on pyroptosis of HCT-116 cells induced by LPS+ATP.Methods The targeting relationship between miR-143-3 p and TLR2 gene was detected by dual luciferase.The pyroptosis model was established after transfection of miR-143-3p mimic.Cell apoptosis was detected by flow cytometry,and the activity of Caspase-1 and LDH was detected by biochemical method.The levels of IL-1β and IL-18 were detected by ELISA.The mRNA levels of NF-κB,TLR2,NLRP3 and GSDMD were detected by q-PCR.The expression of NLRP3 and ASC was detected by immunofluorescence.The protein expressions of TLR2,GS-DMD,p-NF-κB p65 and cleave Caspase-1 were detected by Western blot.Results Dual luciferase assay showed that miR-143-3p targeted TLR2 expression.The expressions of NF-κB,TLR2,NLRP3,GSDMD mRNA and TLR2,GSDMD,p-NF-κB p65,cleave Caspase-1,ASC,NLRP3 protein in miR-143-3p mimic group and si TLR2 group were lower than those in model group.The activity of Caspase-1 and the content of LDH,IL-1β and IL-18 decreased.Conclusion miR-143-3p regulates pyroptosis of ulcerative colitis cells by targeting TLR2 gene and regulating NF-κB/NLRP3/Caspase-1 signaling pathway.
