Emodin upregulates the Sirt2 to attenuate LPS-induced oxidative stress response in RAW264.7 cells
10.3969/j.issn.1006-5725.2024.13.004
- VernacularTitle:大黄素上调Sirt2减轻脂多糖致RAW264.7细胞的氧化应激反应
- Author:
Chun LONG
1
;
Hongying BI
;
Changzhen YANG
;
Jiakai WANG
;
Yan TANG
;
Xu LIU
Author Information
1. 贵州医科大学附属医院重症医学科(贵阳 550004)
- Keywords:
sepsis;
macrophages;
oxidative stress;
emodin;
Sirt2
- From:
The Journal of Practical Medicine
2024;40(13):1785-1790
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to investigate the impact and mechanisms of emodin on oxidative stress response in lipopolysaccharide(LPS)-induced murine mononuclear macrophages(RAW264.7).Methods Involved the use of LPS,RAW264.7 cells,and emodin.Experimental groups included a control group,LPS(1 μg/mL)group,and LPS(1 μg/mL)+emodin(15 μmmol/L)pretreatment group.Aldehyde malondialdehyde(MDA)content,intracellular reactive oxygen species(ROS)levels,and silent information regulator 2(Sirt2)expression were evaluated at 6,12,and 18 hours after LPS exposure.Additionally,RAW264.7 cells were pretreated with Sirt2 inhibitor AGK2(20 μmol/L)followed by LPS stimulation,and the above-mentioned parameters were assessed at 6 hours.Results Compared to the control group,MDA content,ROS levels,Sirt2 mRNA,and protein expression in RAW264.7 cells in the LPS group increased at all time points(all P<0.05).At 6 and 18 hours,MDA content and ROS levels in RAW264.7 cells in the LPS+emodin group decreased significantly(all P<0.05),while at 12 hours,ROS levels were lower in the LPS group compared to the LPS+emodin group(P<0.05).Sirt2 mRNA and protein levels significantly increased at all time points(all P<0.05)compared to the LPS group.In the LPS+emodin+AGK2 group,Sirt2 mRNA and protein levels decreased,and MDA content and ROS levels increased compared to the LPS+emodin group(all P<0.05).Conclusion LPS-induced oxidative stress in RAW264.7 cells and emodin attenuate LPS-induced oxidative stress in RAW264.7 cells through Sirt2.
