Dynamic expression of protein tyrosine phosphatase SHP2 in liver tissue of rats with carbon tetrachloride-induced liver fibrosis
10.3760/cma.j.cn501113-20201231-00692
- VernacularTitle:蛋白酪氨酸磷酸酶SHP2在四氯化碳诱导的肝纤维化大鼠肝组织中的动态表达
- Author:
Lisen HAO
1
;
Jie SONG
;
Rongpeng WU
;
Penglei ZHANG
;
Jingxiu JI
;
Meiyu JIANG
;
Yanbo MO
;
Jing WANG
Author Information
1. 华北理工大学附属医院消化内科，唐山 063000
- Keywords:
Liver cirrhosis;
Gene expression;
SH2 domain-containing protein tyrosine phosphatase 2
- From:
Chinese Journal of Hepatology
2021;29(9):844-848
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the dynamic expression of protein tyrosine phosphatase SHP2 in liver tissue of rats with carbon tetrachloride (CCl 4)-induced liver fibrosis. Methods:Rat liver fibrosis model was established by intraperitoneal injection of CCl 4. Rat liver tissue histopathological changes were detected by HE and Masson-trichrome staining. Immunohistochemical staining, Western blot and real-time fluorescent quantitative PCR were used to detect SHP2 protein and mRNA expression in rat liver tissue. One-way analysis of variance was used for the comparison of means between multiple groups, and the LSD test was used for further inter-group comparison. Results:CCl 4-induced rat liver fibrosis model was successfully constructed, and with the extension of modeling time, the degree of liver fibrosis in rats were aggravated gradually. Immunohistochemical staining results showed that SHP2 was mainly expressed in the cytoplasm of rat liver tissues. With the aggravation of liver fibrosis, the number of cells with positive expression of SHP2 was aggravated gradually ( P < 0.05). Western blot and real-time fluorescent quantitative PCR results showed that the expressions of SHP2 protein and mRNA in rat fibrotic liver tissues at different times in week 2, 4, 6, and 8 were higher in modeling than control group ( P < 0.05), and was aggravated gradually with the liver fibrosis aggravation ( P < 0.05). Conclusion:The expression of SHP2 protein and mRNA in the liver tissue of rats with CCl 4-induced liver fibrosis increased gradually with the degree of liver fibrosis, and the degree of increase was consistent with the degree of liver fibrosis.
