Dapagliflozin inhibits angiotensin Ⅱ-induced cardiomyocyte hypertrophy and apoptosis
10.3969/j.issn.1006-5725.2024.11.010
- VernacularTitle:达格列净抑制血管紧张素Ⅱ诱导的心肌细胞肥大和凋亡
- Author:
Shuan-Gyin SHAO
1
;
Xintong CAI
;
Lili XIAO
;
Lu GAO
Author Information
1. 河南省胸科医院心外科(郑州 450008)
- Keywords:
dapagliflozin;
SGLT2 inhibitor;
angiotensin Ⅱ;
cardiomyocytes;
insulin-like growth factor 1 receptor
- From:
The Journal of Practical Medicine
2024;40(11):1526-1530
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aimed to investigate the effect of Dapagliflozin on the hypertrophy and apoptosis of cardiomyocytes induced by angiotensinⅡ(Ang Ⅱ).Methods Primary rat neonatal cardiomyocytes were isolated,cultured and randomly divided into 4 groups:control group,Ang Ⅱ group,dapagliflozin group 1(0.5 μmol/L),and dapagliflozin group 2(2 μmol/L).α-actin staining was used to detect cell area.qPCR was applied to detect embryonic gene transcription.Tunel staining was adopted to detect cell apoptosis level.The caspase3 kit was used to detect caspase3 activityand western blotting was used to detect classical signal molecules.Results The cell surface area of the Ang Ⅱ group was significantly larger than that of the control group(P＜0.05).The cell surface area of the dapagliflozin group 1 and the dapagliflozin group 2 was significantly lower than that of the Ang Ⅱ group(P＜0.05).The results of qPCR showed that the fetal gene transcription of Ang Ⅱ group was sig-nificantly higher than that of the control group(P＜0.05);the fetal gene transcription of dapagliflozin group 1 and dapagliflozin group 2 was lower than that of Ang Ⅱ group(P＜0.05).Tunel staining showed that the number of apoptosis in the Ang Ⅱ group was higher than that in the control group(P＜0.05);the number of apoptosis in the dapagliflozin group 1 and the Dapagliflozin group 2 was lower than that in the Ang Ⅱ group(P＜0.05).The cas-pase3 activity of the cells in the Ang Ⅱ group was higher than that of the control group(P＜0.05)but lower in dapagliflozin group 1 and the dapagliflozin group 2(P＜0.05).The results of Western blotting showed that the ac-tivation of insulin-like growth factor 1 receptor(IGF1R)and Akt in the Ang Ⅱ group was lower than that in the control group(P＜0.05)but increased in the dapagliflozin group 1 and 2 cells(P＜0.05).Conclusion Dapa-gliflozin could directly act on cardiomyocytes to protect them from Ang Ⅱ-induced damage.
