TMSB10 promotes gastric cancer proliferation and glycolysis based on activation of AMPK/mTOR signal-ing pathway
10.3969/j.issn.1006-5725.2024.11.009
- VernacularTitle:TMSB10促进胃癌细胞增殖及糖酵解:基于激活AMPK/mTOR信号通路
- Author:
Wei WANG
1
;
Xinxin ZHANG
;
Guanghui WANG
;
Jie ZHANG
;
Anran CHEN
;
Jianguang JIA
Author Information
1. 蚌埠医学院第二附属医院肿瘤外科(安徽蚌埠 233040)
- Keywords:
TMSB10;
glycolysis;
gastric cancer;
AMPK/mTOR signaling pathway
- From:
The Journal of Practical Medicine
2024;40(11):1519-1525
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of Thymosinβ10(TMSB10)in gastric cancer and its molecular mechanism in promoting the progression of gastric cancer.Methods We collected pathological sections of 70 patients with gastric cancer in our hospital,detected expression of TMSB10 in gastric adenocarcinoma by immunohistochemistry,and analyzed its prognostic effect.In order to study the mechanism of action,the effects of TMSB10 on the proliferation and glycolysis of gastric cancer cells and its related mechanism were observed by trans-fection technique,CCK8 test,EDU assay,Transwell assay,scratch test,glycolysis assay and Western blot.Results Immunohistochemistry showed that TMSB10 was highly expressed in gastric cancer,while the expression level of TMSB10 was correlated with tumor size(P=0.032),TNM stage(P=0.002)and distant metastasis.In the mechanism study,we found that overexpression of TMSB10 promoted the proliferation,invasion,migration and glycolytic phenotype of gastric cancer cells through in vitro CCK-8 test,EDU assay,Transwel assay and glycolysis assay,and vice versa.Western blot results showed that overexpression of TMSB10 may regulate the occurrence of gastric cancer through up-regulation of the AMPK/mTOR signaling pathway.Conclusions TMSB10 promotes the proliferation,invasion,migration and glycolysis gastric cancer through the AMPK/mTOR signaling pathway.
