Effects of allergens on the expressions of IL-18,IL-18BPa and IL-18Rα in blood CD4+Th1 cells of patients with allergic rhinitis
10.3969/j.issn.1006-5725.2024.11.008
- VernacularTitle:过敏原对过敏性鼻炎患者外周血CD4+Th1 IL-18、IL-18BPa和IL-18Rα表达的影响
- Author:
Junling WANG
1
,
2
,
3
;
Mengmeng ZHAN
;
Fangqiu GU
;
Yifei LI
;
Zhaolong ZHANG
;
Congyi ZHAO
;
Danyang ZHAO
;
Hui ZHENG
;
Yijie ZHANG
;
Bingyu QIN
Author Information
1. 郑州大学人民医院河南省人民医院重症医学部(郑州 450003)
2. 河南大学淮河医院呼吸内科(河南开封 475000)
3. 锦州医科大学附属第一医院变态反应与临床免疫研究中心(辽宁锦州 121001)
- Keywords:
allergic rhinitis;
CD4+Th1 cell;
IL-18;
IL-18Rα
- From:
The Journal of Practical Medicine
2024;40(11):1513-1518
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of allergens on the expressions of IL-18,IL-18BPa and IL-18Rα protein in peripheral blood CD4+Th1 cells of healthy control subjects(HC)and patients with allergic rhi-nitis(AR),and on the expressions of IL-18,IL-18BPa and IL-18Rα mRNA in the peripheral blood CD4+T cells.Methods Blood samples were collected from patients with rhinitis for negative skin prick test(AR-),rhinitis for positive skin prick test(AR+)and HC.Flow cytometry was used to evaluate the effects of allergens on the expres-sions of IL-18,IL-18BPa and IL-18Rα protein in CD4+Th1 cells.The expressions of IL-18,IL-18BPa and IL-18Rα mRNA in CD4+T cells were determined by qPCR.Results Compared with HC,increased IL-18 while de-creased IL-18BPa expressions in Th1 cells of AR-and AR+patients were observed,increased IL-18Rα expression in Th1 cells of AR+patients was also found.Additionally,allergens induced elevated expression of IL-18Rα pro-tein in Th1 cells of HC,and induced elevated mRNA expressions of IL-18,IL-18BPa and IL-18Rα in isolated blood CD4+T cells of AR+patients and HC.Conclusion Allergens may be involved in the pathogenesis of AR by inducing the expressions of IL-18 and IL-18Rα in blood CD4+Th1 cells.
