Proanthocyanidins promotes neurite outgrowth of dorsal root ganglion neurons in rat

VernacularTitle:原花青素对大鼠背根神经节神经元突起生长的影响
Author: Yingxiu CHEN ¹ ; Yingqi GUO ; Huimei ZHANG ; Qi ZHANG ; Jinkun WEN ; Li LUO ; Lixia LI
Author Information

1. 广东药科大学基础医学院(广州 510006)
Keywords: proanthocyanidins; neurons; neurite outgrowth; nerve growth factor
From: The Journal of Practical Medicine 2024;40(10):1357-1363
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of proanthocyanidins(PC)on the neurite outgrowth of rat dorsal root ganglion(DRG)neurons.Methods In vitro,primary rat DRG neurons were cultured wtih a series of concenteation of PC to assess the effect of PC on the number and length of neurites as well as the morphology of growth cone.In vivo,the expression of growth associated protein 43(GAP43)in the early stage of injury was detected using the sciatic nerve crush model.Finally,the impact of PC on nerve growth factor(NGF)expression in DRG neurons was evaluated in vitro using immunofluorescence and ELISA.Results PC significantly increased the number and length of neurites and the number of pseudopodium in growth cones of DRG neurons.PC also promoted the expres-sion of GAP43 in the early stage of sciatic nerve injury in rats and enhanced the expression of NGF in DRG neurons.Conclusion PC may promote the neurite outgrowth by increasing the expression of NGF in DRG neurons.