Methylene blue play a role in preventing septic liver injury by inducing macrophage polarization

VernacularTitle:亚甲蓝通过诱导巨噬细胞极化发挥预防脓毒症肝损伤作用
Author: Han LI ¹ ; Lijun TIAN ; Zhaolian BIAN ; Qiang CHEN ; Lingling SHI ; Xudong HAN
Author Information

1. 南通大学医学院　226001
Keywords: Methylene blue; Sepsis; Liver injury; Macrophage
From: Chinese Journal of Hepatology 2021;29(4):369-372
CountryChina
Language:Chinese
Abstract: Thirty mice were used to establish a sepsis model with cecal ligation and puncture. 15 mg/kg methylene blue or isotonic saline were injected intraperitoneally to observe liver tissue pathological changes. Changes in macrophage frequency and expressional condition of M1 and M2-type hepatic inflammatory factors were detected. After LPS stimulation, the expression level of macrophage inflammatory factor were detected. The results showed that the pathological liver injury was significantly reduced in the MB mice group ( P < 0.05), and the frequency of liver macrophage was not statistically significantly different ( P > 0.05). MB elevation had promoted the expression of M2-type hepatic inflammatory factor ( P < 0.05) and macrophage inflammatory factor ( P < 0.05). MB can play a role in preventing septic liver injury by inducing macrophages polarization to M2-type.