Role of the liver-enriched transcription factor binding site mutation in the C promoter region of hepatitis B virus genome for HBx-enhanced hepatitis B virus replication
10.3760/cma.j.cn501113-20200923-00523
- VernacularTitle:乙型肝炎病毒基因组C区启动子肝富集转录因子结合位点突变对HBx增强乙型肝炎病毒复制的影响
- Author:
Ning HAN
1
;
Libo YAN
;
Lingyao DU
;
Feijun HUANG
;
Hong TANG
Author Information
1. 四川大学华西医院感染性疾病中心，成都 610041
- Keywords:
Hepatitis B virus;
Liver-enriched transcription factor;
Hepatitis B virus x protein;
Site-directed mutagenesis
- From:
Chinese Journal of Hepatology
2021;29(4):350-355
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct a recombinant HBV replication-type plasmid with liver-enriched transcription factor binding site mutation at proximal of HBV C promoter in order to elucidate the role of HBx-enhanced HBV replication.Methods:Site-directed mutagenesis technology was used to construct a recombinant plasmid with liver-enriched transcription factor binding site mutation at proximal of HBV C promoter on the basis of wild-type HBV replicating plasmid and HBV replicating plasmid lacking HBx expression. Subsequently, plasmid transfection was carried out in HBV liver cancer cell replication model and mouse replication model, and HBV replication intermediates of cells and mouse liver tissue were extracted for detection.Results:Based on the HBV replicating plasmid, the HBV replicating plasmid with liver-enriched transcription factor binding site mutation at proximal of HBV C promoter was successfully constructed. HBx-enhanced HBV replication were detected in both the HBV liver cancer replication model and the mouse replication model. After mutating liver-enriched transcription factor binding site mutation at proximal of HBV C promoter, the effect of HBx on the enhancement of HBV replication was not significantly affected.Conclusion:HBx may not enhance HBV replication through liver-enriched transcription factor binding site mutation at proximal of HBV C promoter. The role of other liver-enriched transcription factor binding sites in HBx-enhanced HBV replication needs further study.
