Insulin-like growth factor-1 alleviates hepatocyte senescence by regulating intranuclear p53-progerin pathway
10.3760/cma.j.cn501113-20191213-00459
- VernacularTitle:胰岛素样生长因子-1通过调控核内p53-progerin通路减轻肝细胞衰老
- Author:
Xiaoke JIANG
1
;
Jun LI
;
Yangqiu BAI
;
Hui DING
;
Zhiyu YANG
;
Suofeng SUN
;
Yuan LIANG
;
Cong PENG
;
Shuangyin HAN
;
Xiuling LI
;
Xiaoying LUO
;
Bingyong ZHANG
Author Information
1. 河南省人民医院消化内科 郑州大学人民医院，郑州 450003
- Keywords:
Insulin-like growth factor;
Hepatocyte senescence;
p53;
Progerin
- From:
Chinese Journal of Hepatology
2021;29(3):271-274
- CountryChina
- Language:Chinese
-
Abstract:
To construct cellular senescence model by stimulating primary hepatocytes with hydrogen peroxide (H 2O 2). Primary hepatocytes were transfected with p53 siRNA, progerin siRNA or IGF-1 adenovirus vector. The number of SA-β-Gal stained positive cells and the expression of p53 and progerin were detected. The results showed that p53 siRNA and progerin siRNA had knocked-down the expression of p53 and progerin, and had alleviated the hepatocyte senescence. Transfection of insulin-like growth factor (IGF)-1 adenovirus vector into primary hepatocytes had overexpressed IGF-1, and had alleviated the number of SA-β-Gal-positive cells. The expression of p53 and progerin was down-regulated in the nucleus, while the expression of p53 was up-regulated in the cytoplasm. The co-precipitation and co-localization of p53 and progerin was decreased in the nuclear region of hepatocytes. IGF-1 overexpression can inhibit intranuclear p53 translocation, alleviate the interaction between p53-progerin, and alleviate hepatocyte senescence.
