Preliminary Study of Tissue Engineered Bladder Regeneration with Poly (epsilon-caprolactone) (PCL) Sheet Seeded with Autologous Muscle-derived Stem Cell.
- Author:
Seok Soo BYUN
1
;
Ji Youl LEE
;
Sung Ho GHIL
;
Sang Sub LEE
;
Jin Ho LEE
;
Soon Hong YOOK
;
Dong Keun HAN
;
Hyeon Hoe KIM
;
Eunsik LEE
Author Information
1. Department of Urology, Seoul National University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Bladder;
Atrophy;
Regeneration;
Stem cells
- MeSH:
Animals;
Atrophy;
Cats;
Choline;
Female;
Fluorescent Antibody Technique;
Humans;
Mesentery;
Muscle Cells;
Muscle, Skeletal;
Myosin Heavy Chains;
Rats;
Rats, Sprague-Dawley;
Regeneration*;
Stem Cells*;
Transferases;
Urinary Bladder*
- From:Korean Journal of Urology
2005;46(10):1094-1097
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the feasibility of using a poly (epsilon-caprolactone) (PCL) sheet seeded with autologous muscle-derived stem cells as a bladder substitute. MATERIALS AND METHODS: Muscle-derived stem cells were isolated from the gastrocnemius muscle of 9 female Sprague-Dawley rats using a preplate technique, and cultured on a 5x5mm PCL sheet. The sheets were implanted into the mesentery of the rats in an autologous manner. Three rats were sacrificed 2, 4 and 8 weeks after implantation, and the morphological changes were assessed by H&E and immunofluorescence staining including DAPI, myosin heavy chain (MHC) and choline acetyl transferase (CAT). RESULTS: All the rats survived for the scheduled time. A mild inflammatory reaction was observed around the PCL sheet in the postoperative 2-week specimen but this receded with time. Muscle cells on the sheet were observed over the experimental period. The 8-week specimen showed a moderate amount of muscle cells on the sheet, and MHC and CAT immunofluorescence staining showed a positive reaction. The muscle layer was not well organized. Angiogenesis was quite noticable between the sheet and the muscle cells on the 8-week specimen. CONCLUSIONS: A PCL sheet seeded with autologous muscle-derived stem cells showed skeletal muscle differentiation on the sheets 8 weeks after mesenteric implantation in an autologous manner. This suggests the feasibility of using a PCL sheet seeded with autologous muscle-derived stem cell as a bladder substitute.
