Network pharmacology and molecular docking technology reveal the mechanism of kidney-protecting spirit pill in the treatment of diabetic nephropathy
10.16753/j.cnki.1008-2344.2024.04.005
- VernacularTitle:基于网络药理学与分子对接技术探讨保肾灵丸治疗糖尿病肾病的机制
- Author:
Huijuan LIU
1
;
Yue HU
;
An WANG
;
Fu CAO
;
Shuihong YU
;
Qiguo WU
Author Information
1. 安庆医药高等专科学校药学院,安徽 安庆 246052
- Keywords:
network pharmacology;
molecular docking;
kidney-protecting spirit pill;
diabetic nephropathy
- From:
Journal of Shenyang Medical College
2024;26(4):360-369,375
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of kidney-protecting spirit pill for the treatment of diabetic nephropathy(DN)based on the network pharmacology and molecular docking technology.Methods:The database of TCMSP and Swiss Target Prediction was searched to obtain the active ingredients and targets of kidney-protecting spirit pill,and the intersection with the disease targets was obtained.The protein-protein interaction(PPI)network of intersection targets was constructed,GO and KEGG enrichment were analyzed.The key targets and small molecules were obtained and their interactions were verified by molecular docking.Results:A total of 60 active ingredients and 112 therapeutic DN targets were predicted.The key components were Cerevisterol,3,9-di-O-methylnissolin,Jaranol,Palmidin A and 16α-Hydroxydehydrotrametenolic acid.The key targets were PIK3CA,MAPK1,AKT1,PIK3R1 and BCL2,which were closely related to cancer-related pathways,AGE-RAGE signaling pathway,endocrine resistance,lipids and atherosclerosis pathways in diabetic complications.Conclusion:The mechanism of kidney-protecting spirit pill in the treatment of DN is characterized by multi-components,multi-targets and multi-pathways,with synergistic effects between the herbs,which provides a basis for the study of the pharmacological effects of kidney-protecting spirit pill.
